Comparison of the cytotoxicities ofFusarium metabolites andAlternaria metabolite AAL-toxin to cultured mammalian cell lines
| Autor: | Robert E. Peterson, Ronald F. Vesonder, Helen Gasdorf |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Health
Toxicology and Mutagenesis Metabolite Biology Toxicology Cell Line Mice chemistry.chemical_compound 4-Butyrolactone Fusarium Sphingosine Cricetinae Tumor Cells Cultured medicine Baby hamster kidney cell Animals Cytotoxic T cell Furans Fibroblast Fumonisin B1 Cell Death Chinese hamster ovary cell Alternaria Fusaric Acid General Medicine Mycotoxins Pollution medicine.anatomical_structure chemistry Biochemistry Cell culture Cyclobutanes Fusaric acid |
| Zdroj: | Archives of Environmental Contamination and Toxicology. 24:473-477 |
| ISSN: | 1432-0703 0090-4341 |
| DOI: | 10.1007/bf01146164 |
| Popis: | Four water-soluble Fusarium metabolites (fumonisin B1, fusaric acid, butenolide and moniliformin), water-insoluble pigment (8-O-methylbostrycoidin), and an Alternaria metabolite (AAL-toxin) were tested for relative cytotoxicity to five established mammalian cell lines. Butenolide was the most cytotoxic to all five cell lines. LC50s were; 1 microgram/ml to rat hepatoma (RH) (tumors derived from parenchymal cells), 7 micrograms/ml to baby hamster kidney (BHK-21) fibroblast cells, and 15 micrograms/ml to McCoy mouse (MM) fibroblast cells: LC100s were 1 microgram/ml to Chinese hamster ovary (CHO) fibroblast cells, and 5 micrograms/ml to dog kidney (MDCK) fibroblast cells. Fusaric acid was cytotoxic to the MDCK, MM, RH, and CHO cell lines; moniliformin was cytotoxic to the RH, CHO, and MDCK, cell lines. The pigment, however, was cytotoxic only to RH and CHO cell lines. Fumonisin B1 and a related toxin, AAL-toxin, at a high dose level (100 micrograms/ml) were not cytotoxic to the RH, BHK, MM, CHO and MDCK cell lines. T-2 toxin was used as a positive control, and inhibited all cell lines at the nanogram level. The difference in response of these five cell lines to the toxic metabolites, that were noted in this study, was then used to evaluate nine HPLC fractions obtained from a methanol-water extract of an F. moniliforme culture. The results indicated that this type of cytotoxicity assay may be useful in following the isolation of metabolites from extracts of Fusarium culture, especially F. moniliforme. |
| Databáze: | OpenAIRE |
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