Electrophoretic variants of intracellular catalase of different Candida species

Autor: M.T. Shimizu, Patrícia de Oliveira Carvalho, Natália Reiko Sato Miyasaka, C.S. Unterkircher
Rok vydání: 2004
Předmět:
Zdroj: Mycopathologia. 158(2)
ISSN: 0301-486X
Popis: Intracellular and extracellular catalases of different species of Candida were investigated using different culture media. All the Candida strains produced intracellular catalase, whose enzymatic activity was detected by non-denaturating polyacrylamide gradient (4-30%) gel electrophoresis. The cell extracts presented a major 230 kDa catalase band and in some strains variants of catalase with different molecular weights were detected. Candida catalase activity was not affected by heating at 50 degrees C and incubation with beta-mercaptoethanol, but treatment with sodium dodecyl sulphate inhibited or reduced enzymatic activity. Extracellular enzyme activity was not detected in any of the culture filtrate extracts tested.
Databáze: OpenAIRE