Electrophoretic variants of intracellular catalase of different Candida species
Autor: | M.T. Shimizu, Patrícia de Oliveira Carvalho, Natália Reiko Sato Miyasaka, C.S. Unterkircher |
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Rok vydání: | 2004 |
Předmět: |
Hot Temperature
Veterinary (miscellaneous) Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Mice Species Specificity Candidiasis Oral Enzyme Stability Extracellular Animals Humans Sodium dodecyl sulfate Candida Gel electrophoresis chemistry.chemical_classification Molecular mass biology Virulence Sodium Dodecyl Sulfate Catalase Molecular biology Enzyme assay Culture Media Molecular Weight Enzyme Biochemistry chemistry biology.protein Electrophoresis Polyacrylamide Gel Agronomy and Crop Science Intracellular |
Zdroj: | Mycopathologia. 158(2) |
ISSN: | 0301-486X |
Popis: | Intracellular and extracellular catalases of different species of Candida were investigated using different culture media. All the Candida strains produced intracellular catalase, whose enzymatic activity was detected by non-denaturating polyacrylamide gradient (4-30%) gel electrophoresis. The cell extracts presented a major 230 kDa catalase band and in some strains variants of catalase with different molecular weights were detected. Candida catalase activity was not affected by heating at 50 degrees C and incubation with beta-mercaptoethanol, but treatment with sodium dodecyl sulphate inhibited or reduced enzymatic activity. Extracellular enzyme activity was not detected in any of the culture filtrate extracts tested. |
Databáze: | OpenAIRE |
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