Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid
| Autor: | J B Katz, K M Moser, A D Alstad, G A Gustafson |
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| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Serotype Orbivirus biology Base Sequence Oligonucleotide Molecular Sequence Data Epizootic hemorrhagic disease virus Reoviridae biology.organism_classification Virology Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention Microbiology chemistry.chemical_compound chemistry law Colorimetry Oligonucleotide Probes Polymerase chain reaction DNA Bluetongue virus Research Article |
| Zdroj: | Journal of clinical microbiology. 31(11) |
| ISSN: | 0095-1137 |
| Popis: | A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected 0.01 50% cell culture infectious doses of each serotype of BTV. The sensitivity and serogroup-wide specificity of the PCR-ELOSA may enable it to replace the more expensive, time-consuming, and biohazardous methods used in the identification of BTV. |
| Databáze: | OpenAIRE |
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