Transcriptional regulation of OCI-5/Glypican 3: elongation control of confluence-dependent induction
| Autor: | H K Lee, M. Li, N G Miyamoto, Huiling Yang, Jorge Filmus, R Pullano, R N Buick |
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| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Glypican Transcription Genetic Cellular differentiation Molecular Sequence Data Restriction Mapping Peptide Chain Elongation Translational Biology Transfection Glypican 3 Cell Line Mice Glypicans Transcription (biology) Gene expression Genetics Transcriptional regulation Animals Humans Intestinal Mucosa Promoter Regions Genetic Molecular Biology Regulation of gene expression Base Sequence Promoter Sequence Analysis DNA Molecular biology Rats Up-Regulation Gene Expression Regulation Proteoglycans Heparitin Sulfate Sequence Alignment Heparan Sulfate Proteoglycans |
| Zdroj: | Oncogene. 15(13) |
| ISSN: | 0950-9232 |
| Popis: | OCI-5/Glypican 3, a member of the glypican family of proteoglycans, is the defective gene in the Simpson-Golabi-Behmel overgrowth syndrome. OCI-5 expression is developmentally regulated in the intestinal epithelium, and the mechanism of its regulation was studied in the rat intestinal epithelial cell line IEC-18. A large induction of OCI-5 transcript and protein was observed at high cell density. Among other glypican family members, kappa-glypican also exhibited a confluence-dependent induction in select cell types. Nuclear run-on analysis indicated that cell-density regulation of OCI-5 occurs at the level of transcription. The rat and mouse OCI-5 promoters were cloned and found to be highly conserved, located within CpG islands and contain regions of alternating purine and pyrimidine residues. No TATA-box or recognizable INR element was observed. Consensus binding sites for AP-2, SP-1, zeste and NF-1/CTF are conserved across human, mouse and rat promoters. 5' deletion mapping of the rat promoter identified regions which enhance and repress promoter activity, with no apparent confluence-dependence or tissue-specificity. Nuclear run-on analysis probing different regions of the gene suggests that elongation control plays a role in the induction of OCI-5 by confluence. |
| Databáze: | OpenAIRE |
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