Reagent-saving immunohistochemistry for HER2 using non-contact alternating current electric field mixing
| Autor: | Yoichi Akagami, Kaori Terata, Ryuta Nakamura, Satoru Motoyama, Yoshitaro Saito, Hajime Saito, Satoshi Fujishima, Kazuhiro Imai, Hiroshi Nanjo, Yoshihiro Minamiya, Akiyuki Wakita, Iku Hoshino, Yusuke Sato |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Receptor ErbB-2 Urology Breast Neoplasms HER2 Antibody Sensitivity and Specificity Antibodies Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Breast cancer Electricity Biomarkers Tumor medicine Humans Human Epidermal Growth Factor Receptor 2 Aged Aged 80 and over biology Diagnostic Tests Routine Chemistry Reproducibility of Results General Medicine Middle Aged medicine.disease Immunohistochemistry Staining Dilution 030104 developmental biology 030220 oncology & carcinogenesis Reagent biology.protein Female Antibody |
| Zdroj: | Journal of Clinical Pathology. 72:25-30 |
| ISSN: | 1472-4146 0021-9746 |
| Popis: | AimsHuman epidermal growth factor receptor 2 (HER2)-targeted agents are an effective approach to treating patients with HER2-positive breast cancer. However, the lack of survival benefit in HER2-negative patients, as well as the toxic effects and high cost of the drugs, highlight the need for accurate and prompt assessment of HER2 status. Our aim was to evaluate the clinical utility of a novel reagent-saving immunohistochemistry method (AC-IHC) that saves HER2 antibody by taking advantage of the non-contact mixing effect in microdroplets subjected to an alternating current electric field.MethodsNinety-five specimens were used from patients diagnosed with primary breast cancers identified immunohistochemically as HER2 0/1+, 2+ or 3+ using ASCO/CAP guideline-certified standard IHC. The specimens were all tested using the conventional IHC method (1:50 antibody dilution) as well as AC-IHC (1:50 dilution) and reagent-saving AC-IHC (1:100 dilution).ResultsThe reagent-saving AC-IHC produced stable results with less non-specific staining using smaller amounts of labelled antibody. Moreover, the staining and accuracy of HER2 status evaluated with the reagent-saving AC-IHC method was equal to that achieved with standard IHC.ConclusionsThese results suggest reagent-saving AC-IHC could be used as a clinical tool for accurate and stable HER2 IHC, even when reagent concentrations vary. |
| Databáze: | OpenAIRE |
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