Identification of a rapid mammalian deadenylation-dependent decay pathway and its inhibition by a viral RNA element
Autor: | Joan A. Steitz, Eleanor L. Marshall, Stavroula Mili, Mei Di Shu, Nicholas K. Conrad |
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Rok vydání: | 2005 |
Předmět: |
RNA Stability
Polyadenylation Base Sequence Transcription Genetic Molecular Sequence Data RNA Cell Biology Biology Elements Molecular biology Globins Transcription (biology) In vivo Gene expression Mutation Humans RNA Viral RNA Splice Sites RNA Messenger Signal transduction Molecular Biology Ene reaction Signal Transduction |
Zdroj: | Molecular cell. 24(6) |
ISSN: | 1097-2765 |
Popis: | Cellular RNAs are subject to quality-control pathways that insure the fidelity of gene expression. We previously identified a 79 nt element, the ENE, that is essential for the nuclear accumulation of a viral polyadenylated nuclear (PAN) RNA. Here, we show that intron-less polyadenylated transcripts such as PAN RNA and beta-globin cRNA exhibit two-component exponential decay kinetics in which some transcripts are rapidly degraded (t(1/2) = approximately 15 min) while others decay more slowly (t(1/2) = approximately 3 hr). Inclusion of the ENE protects such transcripts from rapid decay in a poly(A)-dependent fashion. The ENE inhibits deadenylation and decay in nuclear extract and prevents deadenylation of naked RNA by a purified deadenylase, likely through snoRNA-like intramolecular hybridization with the poly(A) tail. The ENE causes increased accumulation of splicing-defective beta-globin pre-mRNAs in vivo. We propose that the ENE-controlled rapid-decay mechanism for polyadenylated transcripts comprises a nuclear pre-mRNA surveillance system in mammalian cells. |
Databáze: | OpenAIRE |
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