Genome Survey Sequencing of Acer truncatum Bunge to Identify Genomic Information, Simple Sequence Repeat (SSR) Markers and Complete Chloroplast Genome
| Autor: | Pan Chang, Ling Zhu, Jinshuan Fan, Lingli Li, Rongkai Wang, Mengran Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Whole genome sequencing Genetics Molecular breeding 0303 health sciences SSR markers Forestry lcsh:QK900-989 Biology biology.organism_classification 01 natural sciences Genome Chloroplast 03 medical and health sciences Acer truncatum Genetic marker genome survey lcsh:Plant ecology Microsatellite chloroplast genome Genome size 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Forests, Vol 10, Iss 2, p 87 (2019) Forests Volume 10 Issue 2 |
| ISSN: | 1999-4907 |
| Popis: | The Acer truncatum Bunge is a particular forest tree species found in the north of China. Due to the recent discovery that its seeds contain a considerable amount of nervonic acid, this species has received more and more attention. However, there have been no reports of the genome in this species. In this study, we report on the Acer truncatum genome sequence produced by genome survey sequencing. In total, we obtained 61.90 Gbp of high-quality data, representing approximately 116x coverage of the Acer truncatum genome. The genomic characteristics of Acer truncatum include a genome size of 529.88 Mbp, a heterozygosis rate of 1.06% and a repeat rate of 48.8%. A total of 392,961 high-quality genomic SSR markers were developed and a graphical map of the annotated circular chloroplast genome was generated. Thus far, this is the first report of de novo whole genome sequencing and assembly of Acer truncatum. We believe that this genome sequence dataset may provide a new resource for future genomic analysis and molecular breeding studies of Acer truncatum. |
| Databáze: | OpenAIRE |
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