Extrachromosomal HIV-1 DNA in Persistently Infected U937 Cells
| Autor: | Pauza Cd, Singh Mk |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
U937 cell
Immunology Extrachromosomal Inheritance Persistently infected Biology Virology Monocytes Cell Line Restriction fragment chemistry.chemical_compound Infectious Diseases Subcloning chemistry Extrachromosomal DNA DNA Viral HIV-1 biology.protein Humans RNA Viral RNA Messenger Hiv 1 dna Genome size DNA |
| Zdroj: | AIDS Research and Human Retroviruses. 6:1027-1030 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.1990.6.1027 |
| Popis: | Persistent human immunodeficiency virus type 1 (HIV-1) infection of U937 monocytic cells resulted in the accumulation of novel forms of extrachromosomal viral DNA. These DNA species are larger than the genome size of HIV-1 and persist indefinitely. The extrachromosomal viral DNA species (E-DNA) were shown to be structurally stable by subcloning of infected cell lines and restriction fragment analysis. Similar E-DNA structures were observed in independent infections. Persistently infected monocytic cells had low levels of viral antigens, reflecting the low levels of viral RNA that were detected. These results support a role for E-DNA in persistent HIV-1 infection of monocytic cells. |
| Databáze: | OpenAIRE |
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