Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Autor: Tatiane Pasquoto-Stigliani, Leonardo Fernandes Fraceto, Renata de Lima, Tais Germano-Costa, Mariana Guilger-Casagrande
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Rok vydání: 2019
Předmět:
Zdroj: Scientific Reports
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
ISSN: 2045-2322
Popis: Submitted by Luana Priscila Costa (luana@reitoria.unesp.br) on 2019-10-10T19:08:29Z No. of bitstreams: 1 casagrande_m_versaoeditor_soro_biosynthesis.pdf: 4370880 bytes, checksum: 5d6b779738c36a413f7cd74037bfb184 (MD5) Approved for entry into archive by Luana Priscila Costa (luana@reitoria.unesp.br) on 2019-10-10T19:09:04Z (GMT) No. of bitstreams: 1 casagrande_m_versaoeditor_soro_biosynthesis.pdf: 4370880 bytes, checksum: 5d6b779738c36a413f7cd74037bfb184 (MD5) Made available in DSpace on 2019-10-10T19:09:04Z (GMT). No. of bitstreams: 1 casagrande_m_versaoeditor_soro_biosynthesis.pdf: 4370880 bytes, checksum: 5d6b779738c36a413f7cd74037bfb184 (MD5) Previous issue date: 2019-10-04 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Biogenic synthesis of silver nanoparticles employing fungi offers advantages, including the formation of a capping from fungal biomolecules, which provides stability and can contribute to biological activity. In this work, silver nanoparticles were synthesized using Trichoderma harzianum cultivated with (AgNP-TS) and without enzymatic stimulation (AgNP-T) by the cell wall of Sclerotinia sclerotiorum. The nanoparticles were evaluated for the control of S. sclerotiorum. The specific activity of the T. harzianum hydrolytic enzymes were determined in the filtrates and nanoparticles. Cytotoxicity and genotoxicity were also evaluated. Both the nanoparticles exhibited inhibitory activity towards S. sclerotiorum, with no new sclerotia development, however AgNP-TS was more effective against mycelial growth. Both the filtrates and the nanoparticles showed specific enzymatic activity. Low levels of cytotoxicity and genotoxicity were observed. This study opens perspectives for further exploration of fungal biogenic nanoparticles, indicating their use for the control of S. sclerotiorum and other agricultural pests. Versão final do editor Universidade Estadual Paulista Julio de Mesquita Filho Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO) 2017/21004-5
Databáze: OpenAIRE
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