Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail

Autor: Josep Domínguez, Adela Cantos, Esther García-García, J. Ruiz-Manzano, Maria Angeles Jimenez, M. L. De Souza-Galvão, Raquel Villar-Hernández, Morten Ruhwald, Israel Molina-Pinargote, Irene Latorre, Beatriz Muriel-Moreno, J. Pilarte, Cristina Prat, Yoel Gonzalez-Diaz, N. Altet, Joan Pau Millet
Přispěvatelé: Institut Català de la Salut, [Villar-Hernández R, Latorre I] Servei de Microbiología, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. Institut d'Investigació Germans Trias i Pujol, Badalona, Spain. CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. Universitat Autònoma de Barcelona, Badalona, Spain. [De Souza-Galvão ML, Jiménez MA] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Ruiz-Manzano J] CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. Universitat Autònoma de Barcelona, Badalona, Spain. Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. [Pilarte J, Cantos A] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Altet N] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. Unidad Clínica de Tratamiento Directamente Observado 'Serveis Clinics', Barcelona, Spain. CIBER de Epidemiología y Salud Pública, Instituto de Salud Carlos III, Badalona, Spain., Vall d'Hebron Barcelona Hospital Campus
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Adult
Male
Tuberculosis
aminoácidos
péptidos y proteínas::péptidos::péptidos y proteínas de señalización intercelular::citocinas::quimiocinas::quimiocinas CXC::quimiocina CXCL10 [COMPUESTOS QUÍMICOS Y DROGAS]
Otros calificadores::/diagnóstico [Otros calificadores]
Tuberculosi - Diagnòstic
lcsh:Medicine
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Article
QuantiFERON
03 medical and health sciences
0302 clinical medicine
Latent Tuberculosis
Other subheadings::/diagnosis [Other subheadings]
medicine
Humans
Postal Service
Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis::Latent Tuberculosis [DISEASES]
lcsh:Science
Whole blood
Multidisciplinary
Chromatography
Latent tuberculosis
Filter paper
Spots
business.industry
infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias grampositivas::infecciones por Actinomycetales::micobacteriosis::tuberculosis::tuberculosis latente [ENFERMEDADES]
técnicas de investigación::métodos epidemiológicos::rastreo de contactos [TÉCNICAS Y EQUIPOS ANALÍTICOS
DIAGNÓSTICOS Y TERAPÉUTICOS]
lcsh:R
Infection diagnosis
Plasma
medicine.disease
Quimiocines
Chemokine CXCL10
030104 developmental biology
Amino Acids
Peptides
and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines
CXC::Chemokine CXCL10 [CHEMICALS AND DRUGS]
ROC Curve
Cytokines
Female
lcsh:Q
Dried Blood Spot Testing
Investigative Techniques::Epidemiologic Methods::Contact Tracing [ANALYTICAL
DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT]
Contact Tracing
business
030217 neurology & neurosurgery
Interferon-gamma Release Tests
Zdroj: Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
instname
Scientia
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
ISSN: 2045-2322
DOI: 10.1038/s41598-019-40778-1
Popis: The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
Databáze: OpenAIRE
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