Resistance of the Manila clam (Venerupis philippinarum) to infection with Mikrocytos mackini
| Autor: | Susan M. Bower, Geoff Lowe, Gary R. Meyer, Sarah C. Davies |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Mikrocytos mackini
Time Factors biology Ecology Hybridization probe DNA Protozoan biology.organism_classification Bivalvia Ostreidae Immunity Innate Microbiology Eukaryotic Cells Venerupis philippinarum Parasite hosting Crassostrea Animals Disease Susceptibility Mollusca Pathogen Protozoan Infections Animal Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of invertebrate pathology. 98(1) |
| ISSN: | 1096-0805 |
| Popis: | Manila clams ( Venerupis philippinarum ) challenged in laboratory trials via bath exposure proved to be resistant to infections with Mikrocytos mackini (protistan parasite of unknown taxonomic affiliation), while Pacific oysters ( Crassostrea gigas ) challenged simultaneously using identical conditions developed infections. Although M. mackini was detected by a nucleic acid pathogen specific (PCR) assay in 10–30% of the challenged V. philippinarum that were sampled soon after exposure (0–48 h, n = 40), all of the subsequent V. philippinarum ( n = 62) sampled 9–17 weeks post-exposure tested negative for M. mackini by PCR assay. Prevalence of infection for the exposed C. gigas ( n = 100) during this same period ranged from 50% to 100% by PCR assay. Infection was confirmed in the oysters (58%, n = 60) by a digoxigenin-labelled DNA probe designed to detect M. mackini by in situ hybridization, but M. mackini was not found in any of the exposed Manila clams ( n = 63) using this technique. |
| Databáze: | OpenAIRE |
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