Deoxyribonuclease-Inhibitory antibodies in systemic lupus erythematosus
| Autor: | Trai-Ming Yeh, Hung-Chuan Chang, Chun-Ching Liang, Jiunn-Jong Wu, Ming-Fei Liu |
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| Rok vydání: | 2003 |
| Předmět: |
Deoxyribonucleases
Streptococcus pyogenes Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Mice Inbred Strains Cell Biology General Medicine Urine Kinetics Mice Proteinuria Reference Values Antibodies Antinuclear Animals Deoxyribonuclease I Humans Lupus Erythematosus Systemic Cattle Female Pharmacology (medical) Molecular Biology |
| Zdroj: | Journal of Biomedical Science. 10:544-551 |
| ISSN: | 1423-0127 1021-7770 |
| DOI: | 10.1007/bf02256116 |
| Popis: | Deoxyribonucleases (DNases) are key enzymes for digesting DNA. Abnormalities in the function of these enzymes may contribute to the development of anti-DNA antibodies in systemic lupus erythematosus (SLE). In this study, we used bovine DNase 1-coated ELISA plates to screen anti-DNase antibodies in SLE patients. About 62% of the sera of SLE patients (63/101) were positive for anti-DNase antibodies compared to only 8% of normal controls (8/98). A positive correlation was also found between the concentrations of anti-DNase and anti-DNA antibodies in sera of SLE patients. Affinity-purified anti-DNase immunoglobulin G (IgG) from pooled sera of SLE patients bound to bovine DNase as well as DNA. A synthetic peptide, corresponding to the catalytic site of DNase, was able to completely inhibit the binding of anti-DNase IgG to DNase. In addition to bovine DNase, the anti-DNase IgG also bound to and inhibited the enzymatic activities of DNase present in streptococcal supernatants and human urine. Immunization of lupus-prone NZB/NZW mice with bovine DNase enhanced the production of anti-DNase and DNA antibodies, and accelerated the occurrence of proteinuria. Taken together, these results suggest that DNase-inhibitory antibodies which recognize a conserved epitope near the catalytic site of DNase may act in the pathogenesis of SLE. |
| Databáze: | OpenAIRE |
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