Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma
Autor: | Yasuteru Muragaki, Itaru Tojyo, Shigeyuki Fujita, Shin-ichi Murata, Fuyuki Sato, Ujjal K. Bhawal |
---|---|
Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Cancer Research Pathology medicine.medical_specialty Occludin Biochemistry 03 medical and health sciences 0302 clinical medicine SOX2 Cell Line Tumor Proliferating Cell Nuclear Antigen Biomarkers Tumor Genetics medicine Carcinoma Humans Claudin-4 Basaloid Squamous Cell Carcinoma Molecular Biology Aged Neoplasms Basal Cell Aged 80 and over biology Chemistry SOXB1 Transcription Factors Cancer Middle Aged Cell cycle medicine.disease Immunohistochemistry Proliferating cell nuclear antigen stomatognathic diseases 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Carcinoma Squamous Cell biology.protein Molecular Medicine Female |
Zdroj: | Molecular Medicine Reports. |
ISSN: | 1791-3004 1791-2997 |
Popis: | Basaloid squamous cell carcinomas (BSCCs) in oral lesions are extremely rare, and the histology is not well understood. Histologically, they are often similar to conventional squamous cell carcinoma (SCC). The present study was designed with an aim to distinguish BSCC from SCC using claudin‑4, occludin, SRY‑box 2 (SOX2) and proliferating cell nuclear antigen (PCNA) immunoreactivities and staining patterns. Three BSCCs (with abundant, with moderate, and without squamous components) specimens and 20 SCC specimens were selected for comparison of their immunoreactivity. These specimens were stained with claudin‑4, occludin, SOX2 and PCNA. In addition to histological analysis, the expression of claudin‑4, occludin and PCNA was determined in oral cancer HSC2 and HSC3 cells with or without SOX2 overexpression, and cell proliferation was determined by XTT assay. Claudin‑4 had strong and occludin had weak immunoreactivity as detected in the membrane of squamous components of BSCC but not in cancer cells. No obvious detection of squamous components and cancer cells were observed in SCC. SOX2 and PCNA immunoreactivities in SCC had dot‑like staining patterns in the nuclei of partial and marginal cancer cells. In contrast, in BSCCs, SOX2 and PCNA had diffuse staining patterns in almost all cancer cells. SOX2 overexpression had little effect on the expression levels of claudin‑4, occludin and PCNA. It also had little effect on the cell proliferation of HSC2 and HSC3 cells. Differences in immunoreactivity and staining pattern may be valuable to distinguish between BSCC and SCC in diagnosis. |
Databáze: | OpenAIRE |
Externí odkaz: |