Antithrombotic Effects of Endocardial Endothelial Cells-Comparison with Coronary Artery Endothelial Cells
| Autor: | Seishi Nosaka, Kengo Nakayama, Katsuhiro Tamura, Tetsuya Sasaki, Tomoki Hanada, Yuhei Saitoh, Masanobu Yamauchi, Michio Hashimoto, Kwansong Ku, Sumio Masumura |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Platelet Aggregation Endothelium Swine Indomethacin Alpha (ethology) Cell Count Prostacyclin 6-Ketoprostaglandin F1 alpha Pharmacology Biochemistry Endocrinology Thrombin Transforming Growth Factor beta Internal medicine Animals Medicine Platelet Platelet activation Enzyme Inhibitors Incubation Cells Cultured Platelet-Derived Growth Factor biology business.industry Epoprostenol stomatognathic diseases NG-Nitroarginine Methyl Ester medicine.anatomical_structure cardiovascular system biology.protein Cardiology lipids (amino acids peptides and proteins) Endothelium Vascular business Platelet-derived growth factor receptor Endocardium medicine.drug |
| Zdroj: | Prostaglandins. 53:305-319 |
| ISSN: | 0090-6980 |
| DOI: | 10.1016/s0090-6980(97)00039-7 |
| Popis: | The purpose of this study was to assess the anti-platelet properties of endocardial endothelial cells (EECs) by measuring platelet aggregation after a brief incubation with cultured EECs. EECs were isolated from the right ventricles of porcine hearts and coronary artery endothelial cells (C-ECs) were also isolated from the same animals. After brief incubations (2-min) of platelet suspensions with cultured EEC and CEC monolayers, platelet aggregation in response to thrombin and 6-keto-PGF1 alpha (a stable metabolite of PGI2) content of platelet suspensions were measured. Platelet aggregation was significantly inhibited by a brief incubation of platelet suspensions with EEC and C-ECs monolayers. Pretreatment of EECs and C-ECs with indomethacin (5 x 10(-5) M) restored platelet activity, but pretreatment with N omega-nitro-L-arginine methyl ester (L-NAME, 5 x 10(-5) M) or hemoglobin (1 x 10(-6) M) did not. Platelet/EEC interactions multiplicatively increased the 6-keto-PGF1 alpha content of platelet suspensions and the 6-keto-PGF1 alpha content of platelet suspensions after incubations with EECs correlated significantly with the inhibition of platelet aggregation. Both the anti-aggregation properties and 6-keto-PGF1 alpha production were significantly greater in EECs than in C-ECs. A brief incubation (2-min) with PDGF (10 ng/ml) or TGF-beta (1 and 10 ng/ml) stimulated 6-keto-PGF1 alpha production in EECs but not in C-ECs, although these growth factors stimulated 6-keto-PGF1 alpha production in C-ECs after a longer incubation time (30 or 60 min). In this study, after a brief incubation (2-min) with platelet suspensions, EECs inhibited platelet aggregation mainly through the release of PGI2 but not EDRF. As this anti-aggregation property was significantly greater in EECs than in C-ECs, it is suggested that endocardial endothelial PGI2 may inhibit both intracardiac and intracoronary artery thrombus formation, contributing to the prevention of myocardial ischemia. |
| Databáze: | OpenAIRE |
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