Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19
| Autor: | Bing Chen, Jun Zhang, Timothy M. Caradonna, Edward T. Ryan, Tal Gilboa, David R. Walt, Aaron G. Schmidt, Roey Lazarovits, Limor Cohen, Evan L. Busch, Maia Norman, Blake M. Hauser, Chih Ping Mao, Alana F. Ogata, Jared Feldman, Adam M. Maley, Yongfei Cai, Richelle C. Charles, Galit Alter |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Biomedical Engineering Medicine (miscellaneous) Bioengineering Sensitivity and Specificity Immunoglobulin G Article Antibodies Serology 03 medical and health sciences 0302 clinical medicine Antigen medicine Humans Seroconversion Pandemics Aged Aged 80 and over Immunoassay medicine.diagnostic_test biology business.industry SARS-CoV-2 Outbreak Nucleic acid test COVID-19 Diagnostic markers Middle Aged Virology Reverse transcriptase Computer Science Applications 030104 developmental biology Viral infection biology.protein Antibody business 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Nature Biomedical Engineering |
| ISSN: | 2157-846X |
| DOI: | 10.1038/s41551-020-00611-x |
| Popis: | Sensitive assays are essential for the accurate identification of individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we report a multiplexed assay for the fluorescence-based detection of seroconversion in infected individuals from less than 1 µl of blood, and as early as the day of the first positive nucleic acid test after symptom onset. The assay uses dye-encoded antigen-coated beads to quantify the levels of immunoglobulin G (IgG), IgM and IgA antibodies against four SARS-CoV-2 antigens. A logistic regression model trained using samples collected during the pandemic and samples collected from healthy individuals and patients with respiratory infections before the first outbreak of coronavirus disease 2019 (COVID-19) was 99% accurate in the detection of seroconversion in a blinded validation cohort of samples collected before the pandemic and from patients with COVID-19 five or more days after a positive nasopharyngeal test by PCR with reverse transcription. The high-throughput serological profiling of patients with COVID-19 allows for the interrogation of interactions between antibody isotypes and viral proteins, and should help us to understand the heterogeneity of clinical presentations. A multiplexed fluorescence-based assay detects seroconversion in individuals infected with SARS-CoV-2 from less than 1 µl of blood as early as the day of the first positive nasopharyngeal nucleic acid test after symptom onset. |
| Databáze: | OpenAIRE |
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