Modified RNA sequence pools forin vitroselection
| Autor: | Yun Lin, Stanley Gill, Qiu Qiu, Sumedha Jayasena |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Base Sequence
Pancreatic Elastase RNase P Molecular Sequence Data Ribonuclease T1 RNA Nuclease protection assay Hydrogen-Ion Concentration Biology Ligands Cleavage (embryo) In vitro Kinetics Radioligand Assay Biochemistry Genetics Humans Nucleic Acid Conformation Amino Acid Sequence Leukocyte Elastase Pancreatic elastase Peptide sequence |
| Zdroj: | Nucleic Acids Research. 22:5229-5234 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/22.24.5229 |
| Popis: | We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure. |
| Databáze: | OpenAIRE |
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