An easy and low-cost biomagnetic methodology to study regional gastrointestinal transit in rats
| Autor: | Armênio Aguiar dos Santos, André G. Próspero, Leonardo de Barros Pinto, Guilherme Soares, Fabiano Carlos Paixão, José Ricardo de Arruda Miranda, Erick G. Stoppa, Ricardo Puziol de Oliveira, Gabriel Gustavo de Albuquerque Biasotti |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), Universidade de São Paulo (USP), Federal University of Ceará |
| Rok vydání: | 2021 |
| Předmět: |
Diagnostic Imaging
Biomedical Engineering Alternate current Magnetics 03 medical and health sciences chemistry.chemical_compound Cecum 0302 clinical medicine Animals Medicine Alternate current biosusceptometry Gastrointestinal Transit 030304 developmental biology Test meal Phenol red 0303 health sciences Gastric emptying business.industry Stomach Gastrointestinal transit Magnetic imaging Small intestine Rats medicine.anatomical_structure Gastric Emptying chemistry 030211 gastroenterology & hepatology business Biomedical engineering |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1862-278X 0013-5585 |
| DOI: | 10.1515/bmt-2020-0202 |
| Popis: | Made available in DSpace on 2022-04-29T08:29:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 The identification of gastrointestinal (GI) motility disorders requires the evaluation of regional GI transit, and the development of alternative methodologies in animals has a significant impact on translational approaches. Therefore, the purpose of this study was to validate an easy and low-cost methodology (alternate current biosusceptometry-ACB) for the assessment of regional GI transit in rats through images. Rats were fed a test meal containing magnetic tracer and phenol red, and GI segments (stomach, proximal, medial and distal small intestine, and cecum) were collected to assess tracer's retention at distinct times after ingestion (0, 60, 120, 240, and 360 min). Images were obtained by scanning the segments, and phenol red concentration was determined by the sample's absorbance. The temporal retention profile, geometric center, gastric emptying, and cecum arrival were evaluated. The correlation coefficient between methods was 0.802, and the temporal retention of each segment was successfully assessed. GI parameters yielded comparable results between methods, and ACB images presented advantages as the possibility to visualize intrasegmental tracer distribution and the automated scan of the segments. The imaging approach provided a reliable assessment of several parameters simultaneously and may serve as an accurate and sensitive approach for regional GI research in rats. Department of Biophysics and Pharmacology São Paulo State University Biosciences Institute of Botucatu, Prof. Antonio Celso Wagner Zanin Street Science and Technology Institute Federal University of São Paulo Department of Physiology and Pharmacology Federal University of Ceará Ribeirão Preto Medical School São Paulo University Department of Biophysics and Pharmacology São Paulo State University Biosciences Institute of Botucatu, Prof. Antonio Celso Wagner Zanin Street |
| Databáze: | OpenAIRE |
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