The total peroxyl radical trapping potential in serum - an assay to define the stage of atherosclerosis
| Autor: | Camelia S. Stancu, Daniela Toporan, Anca Sima, Maya Simionescu, Loredan S. Niculescu |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Lipid Peroxides medicine.medical_specialty Antioxidant medicine.medical_treatment Radical Coronary Artery Disease medicine.disease_cause Antioxidants Article Mice Cricetinae Internal medicine medicine Animals Humans Myocardial infarction Stage (cooking) Triglycerides Mesocricetus business.industry Muscle Smooth Cell Biology medicine.disease Coronary heart disease Peroxides Rats Cholesterol Endocrinology Diabetes Mellitus Type 2 Aortic Valve Peroxyl radicals Immunology Molecular Medicine Rabbits business Ex vivo Oxidative stress |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Lipid peroxides were identified among the factors that contribute to the atherosclerotic plaque formation in the arterial wall. We hypothesised that a correlation may exist between the content of antioxidant constituents in the serum and the gravity of atherosclerosis. To this purpose, we have determined the serum total peroxyl radical‐ trapping potential (TRAP), which is the combined capacity of all antioxidants to neutralize free radicals in serum and followed its variation in hyperlipemic animals in correlation with the stage of atherosclerosis. In addition, we compared TRAP values in the sera of coronary heart disease (CHD) patients, with or without type II diabetes mellitus. Results showed that after 18 weeks of hyperlipemic diet, the mean TRAP values measured in sera isolated from hyperlipemic hamsters exhibited an about 44% decrease, in good agreement with the increase of serum cholesterol and triglycerides. In the 3 groups of CHD patients, TRAP values decreased with about 10% in sera of stable angina patients, 20% in unstable patients, as compared with normal subjects. The lowest TRAP values were detected in the sera of patients with acute myocardial infarction. The results obtained for different experimental animals and for CHD patients sera indicate that the TRAP method, as adapted in our laboratory, is a reliable and reproducible assay, fit to be used in clinical studies as an ex vivo measurable parameter that correlates with the stage of the atherosclerosis. |
| Databáze: | OpenAIRE |
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