Controlling the production of phytotoxin pyriculol in Pyricularia oryzae by aldehyde reductase
| Autor: | Toshiaki Hayashi, Hiroyuki Osada, Yuuki Furuyama, Hiromasa Kiyota, Hiroshi Hirota, Takashi Kamakura, Takayuki Motoyama, Toshihiko Nogawa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pyricularia Stereochemistry Alcohol Reductase 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Aldehyde Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Biosynthesis Ascomycota Aldehyde Reductase Molecular Biology chemistry.chemical_classification biology 010405 organic chemistry Organic Chemistry General Medicine Phytotoxin Mycotoxins biology.organism_classification 0104 chemical sciences 030104 developmental biology chemistry Alcohol oxidation Benzaldehydes Fatty Alcohols Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 85(1) |
| ISSN: | 1347-6947 |
| Popis: | Pyricularia oryzae is one of the most devastating plant pathogens in the world. This fungus produces several secondary metabolites including the phytotoxin pyriculols, which are classified into 2 types: aldehyde form (pyriculol and pyriculariol) and alcohol form (dihydropyriculol and dihydropyriculariol). Although interconversion between the aldehyde form and alcohol form has been predicted, and the PYC10 gene for the oxidation of alcohol form to aldehyde is known, the gene responsible for the reduction of aldehyde to alcohol form is unknown. Furthermore, previous studies have predicted that alcohol analogs are biosynthesized via aldehyde analogs. Herein, we demonstrated that an aldo/keto reductase PYC7 is responsible for the reduction of aldehyde to alcohol congeners. The results indicate that aldehyde analogs are biosynthesized via alcohol analogs, contradicting the previous prediction. The results suggest that P. oryzae controls the amount of pyriculol analogs using two oxidoreductases, PYC7 and PYC10, thereby controlling the bioactivity of the phytotoxin. |
| Databáze: | OpenAIRE |
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