Expression of Defective Hepatitis B Virus Particles Derived from Singly Spliced RNA Is Related to Liver Disease

Autor: Stanislas Pol, Jonathan Pol, Philippe Bonnard, Bertrand Nalpas, Véronique Schneider, Florianne Garreau, Patrick Soussan, Karine Lacombe, Dina Kremsdorf, Catherine Le Pendeven
Přispěvatelé: Pathogenèse des Virus de l'Hépatite B (PVHB), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Université Pierre et Marie Curie - Paris 6 (UPMC), Service de virologie [Hôpital Tenon], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département de Virologie - Department of Virology, Institut Pasteur [Paris], Service d'Immunologie [Paris], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département d'hépatologie [CHU Cochin], Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Cochin [AP-HP], Services des Maladies Infectieuses et Tropicales [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service des maladies infectieuses et tropicales [CHU Tenon], Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale, Agence Nationale de Recherche sur le SIDA et les Hépatites Virales (grant A020042 to the study), French Research Ministry (grant to J.P.), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM), POL, Jonathan, Service de Maladies infectieuses et tropicales [CHU Tenon]
Rok vydání: 2008
Předmět:
Liver Cirrhosis
liver diseases
viruses
dna
MESH: RNA
Viral/genetics

medicine.disease_cause
MESH: RNA Splicing
MESH: Genotype
Liver disease
0302 clinical medicine
Orthohepadnavirus
MESH: DNA
Viral/genetics

Immunology and Allergy
rna
MESH: Hepatitis B virus/pathogenicity
MESH: Hepatitis B/genetics
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
hepatitis b virus dna
MESH: Statistics
Nonparametric

0303 health sciences
MESH: DNA
Viral/blood

Defective Viruses
virus diseases
Viral Load
Hepatitis B
3. Good health
Infectious Diseases
HBeAg
Hepatocellular carcinoma
Carrier State
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA
Viral

030211 gastroenterology & hepatology
MESH: Genome
Viral

MESH: Carrier State
MESH: Viral Load
Hepatitis B virus
Genotype
RNA Splicing
MESH: Inflammation/virology
MESH: Defective Viruses/pathogenicity
Genome
Viral

Biology
digestive system
Statistics
Nonparametric

Hepatitis B virus PRE beta
Virus
Necrosis
03 medical and health sciences
MESH: Hepatitis B/virology
hepatitis b virus liver
medicine
Humans
hepatitis b virus duck
030304 developmental biology
Inflammation
MESH: Necrosis
MESH: Humans
MESH: Liver Cirrhosis/pathology
biochemical phenomena
metabolism
and nutrition

biology.organism_classification
medicine.disease
Virology
digestive system diseases
Hepadnaviridae
MESH: Hepatitis B virus/genetics
DNA
Viral

MESH: Defective Viruses/genetics
Immunology
MESH: Inflammation/pathology
MESH: Liver Cirrhosis/virology
Zdroj: Journal of Infectious Diseases
Journal of Infectious Diseases, Oxford University Press (OUP), 2008, 198 (2), pp.218-25. ⟨10.1086/589623⟩
Journal of Infectious Diseases, 2008, 198 (2), pp.218-25. ⟨10.1086/589623⟩
ISSN: 1537-6613
0022-1899
DOI: 10.1086/589623
Popis: Background. Defective hepatitis B virus (HBV) particles, generated from singly spliced HBV RNA, have been detected in chronic carriers of HBV. The present study was designed to quantify the expression of defective HBV (dHBV) and wild-type HBV (wtHBV) genomes in the serum of patients with HBV infection and its relation to the severity of liver disease. Methods. HBV and dHBV loads were determined by quantitative polymerase chain reaction in the serum of 89 untreatedHBV-infectedpatients(31coinfectedwithhumanimmunodeficiencyvirus[HIV]type1)withliverdisease of different stages. The ratio of dHBV DNA to total (wtHBV plus dHBV) HBV DNA (dHBV/HBV ratio) was used to express data independently of the level of viral replication. Results. DespiteaglobalcorrelationbetweendHBVandwtHBVload,thedHBV/HBVratiorangedfrom0.001% to 69%. The variation in dHBV/HBV ratio was independent of HIV coinfection, HBV genotype, and precore mutations. The mean dHBV/HBV ratio was higher in patients with severe liver necrosis and fibrosis. Conclusions. OurdataindicatethatanelevateddHBV/HBVratioisassociatedwithlivernecroinflammationand fibrosis disease, suggesting a regulation of dHBV expression according to the severity of the liver disease. The dHBV/ HBV ratio may help to better define liver disease stage during HBV infection. Persistent human hepatitis B virus (HBV) infection is a major public health problem. The major complications of chronic HBV infection are the development of liver cirrhosis and hepatocellular carcinoma (HCC) [1‐3]. Evidence indicating a direct involvement of HBV in this process is now available [3]; indeed, recent data have demonstrated that serum HBV DNA levels are strong predictorsoftheriskofHCC,independentofhepatitisB e antigen (HBeAg) expression, serum alanine amino
Databáze: OpenAIRE