Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism
| Autor: | Duethorn, Binyamin, Groll, Fabian, Rieger, Bettina, Drexler, Hannes CA, Brinkmann, Heike, Kremer, Ludmila, Stehling, Martin, Borowski, Marie-Theres, Mildner, Karina, Zeuschner, Dagmar, Zernicka-Goetz, Magdalena, Stemmler, Marc P, Busch, Karin B, Vaquerizas, Juan M, Bedzhov, Ivan |
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| Přispěvatelé: | Drexler, Hannes CA [0000-0002-8285-0644], Borowski, Marie-Theres [0000-0003-3648-7757], Zeuschner, Dagmar [0000-0002-6712-0192], Zernicka-Goetz, Magdalena [0000-0002-7004-2471], Stemmler, Marc P [0000-0002-7866-3686], Vaquerizas, Juan M [0000-0002-6583-6541], Bedzhov, Ivan [0000-0002-2122-6485], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
Male
Pluripotent Stem Cells Science 631/136/2086 General Physics and Astronomy 631/532/2064 Embryonic Development 631/80/642/333/1465 13/109 Membrane trafficking 13 14 96/31 General Biochemistry Genetics and Molecular Biology Article 38 Mice 13/100 Animals 96/2 Embryonic Stem Cells Cell Proliferation 42 64 Multidisciplinary 631/80/313 631/136/532/2064 82/58 General Chemistry Energy metabolism Cytoskeletal Proteins Blastocyst 14/63 Embryogenesis 38/77 38/39 64/60 Female |
| Zdroj: | Nature Communications Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022) |
| ISSN: | 2041-1723 |
| Popis: | Lima1 is an extensively studied prognostic marker of malignancy and is also considered to be a tumour suppressor, but its role in a developmental context of non-transformed cells is poorly understood. Here, we characterise the expression pattern and examined the function of Lima1 in mouse embryos and pluripotent stem cell lines. We identify that Lima1 expression is controlled by the naïve pluripotency circuit and is required for the suppression of membrane blebbing, as well as for proper mitochondrial energetics in embryonic stem cells. Moreover, forcing Lima1 expression enables primed mouse and human pluripotent stem cells to be incorporated into murine pre-implantation embryos. Thus, Lima1 is a key effector molecule that mediates the pluripotency control of membrane dynamics and cellular metabolism. How pluripotency transcription factors regulate the cellular architecture and energetics has remained largely unknown. Here the authors identify Lima1 as a key effector that mediates the pluripotency control of membrane dynamics and cellular metabolism. |
| Databáze: | OpenAIRE |
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