The autotransporter protein from Bordetella avium, Baa1, is involved in host cell attachment
| Autor: | David M. Miyamoto, Rachel C. Fernandez, H. Kuzmiak-Ngiam, Larissa K. Temple, Stephanie B. Stockwell, Nathan M. Beach |
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| Rok vydání: | 2011 |
| Předmět: |
Turkeys
Gram-negative bacteria Bordetella avium Virulence Microbiology Article Bacterial Adhesion Virulence factor 03 medical and health sciences Bacterial Proteins Animals Pathogen Poultry Diseases Bordetella Infections 030304 developmental biology 0303 health sciences biology 030306 microbiology biology.organism_classification Virology Bacterial adhesin Autotransporter domain Autotransporters |
| Zdroj: | Microbiological Research. 167:55-60 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2011.04.003 |
| Popis: | Bordetella avium is a Gram negative upper respiratory tract pathogen of birds. B. avium infection of commercially raised turkeys is an agriculturally significant problem. Here we describe the functional analysis of the first characterized B. avium autotransporter protein, Baa1. Autotransporters comprise a large family of proteins found in all groups of Gram negative bacteria. Although not unique to pathogenic bacteria, autotransporters have been shown to perform a variety of functions implicated in virulence. To test the hypothesis that Baa1 is a B. avium virulence factor, unmarked baa1 deletion mutants (Δbaa1) were created and tested phenotypically. It was found that baa1 mutants have wild-type levels of serum sensitivity and infectivity, yet significantly lower levels of turkey tracheal cell attachment in vitro. Likewise, semi-purified recombinant His-tagged Baa1, expressed in Escherichia coli, was shown to bind specifically to turkey tracheal cells via western blot analysis. Taken together, we conclude that Baa1 acts as a host cell attachment factor and thus plays a role B. avium virulence. |
| Databáze: | OpenAIRE |
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