Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element
| Autor: | Kathleen E. McGinness, Gerald F. Joyce |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular Transcription Genetic Molecular Sequence Data Oligonucleotides RNA-dependent RNA polymerase RNA polymerase II Crystallography X-Ray Biochemistry Viral Proteins medicine RNA polymerase I T7 RNA polymerase Promoter Regions Genetic Molecular Biology RNA polymerase II holoenzyme Polymerase Base Sequence biology DNA-Directed RNA Polymerases Cell Biology Ribonucleotides Molecular biology Mutagenesis Site-Directed biology.protein Nucleic Acid Conformation Transcription factor II D Small nuclear RNA medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 277:2987-2991 |
| ISSN: | 0021-9258 |
| Popis: | A systematic analysis was carried out to examine the effects of ribonucleotide substitution at various locations within the promoter element for T7 RNA polymerase. Ribonucleotides could be introduced at most positions without significantly decreasing transcription efficiency. A critical window of residues that were intolerant of RNA substitution was defined for both the non-template and template strands of the promoter. These residues are involved in important contacts with the AT-rich recognition loop, specificity loop, and P-intercalating hairpin of the polymerase. These results highlight the malleability of T7 RNA polymerase in recognizing its promoter element and suggest that promoters with altered backbone conformations may be used in molecular biology applications that employ T7 RNA polymerase for in vitro transcription. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|