Corosolic acid induces potent anti-cancer effects in CaSki cervical cancer cells through the induction of apoptosis, cell cycle arrest and PI3K/Akt signalling pathway
| Autor: | Jian Hai Zhang, Yong Qian Xu, Xing Sheng Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Apoptosis Biology Cell cycle arrest 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cervical carcinoma CaSki cervical cancer cell Protein kinase B PI3K/AKT/mTOR pathway Pharmacology Akt signalling Anti-cancer lcsh:RM1-950 Corosolic acid PI3K/Akt signalling Molecular biology Cell biology 030104 developmental biology lcsh:Therapeutics. Pharmacology chemistry 030220 oncology & carcinogenesis Cellular Morphology |
| Zdroj: | Bangladesh Journal of Pharmacology, Vol 11, Iss 2 (2016) Bangladesh Journal of Pharmacology, Vol 11, Iss 2, Pp 453-459 (2016) |
| ISSN: | 1991-0088 |
| Popis: | The main objective of the present study was to investigate the anti-tumor activity of corosolic acid in CaSki human cervical cancer cells. Fluorescence and phase contrast microscopic techniques were used to study the effect of the compound on cellular morphology and apoptosis. Results revealed that corosolic acid exerted potent, dose- and time-dependent growth inhibitory effects in CaSki cell proliferation. Cells got detached from one another making clusters of small number of cells floating in the medium. After the cells were treated with 10, 50 and 100 µM concentrations of corosolic acid, cells began to emit orange red fluorescence more heavily at the centre of cells indicating apoptosis. Corosolic acid also induced G2/M cell cycle arrest in a dose-dependent manner. Increasing doses of corosolic acid treatment to these cells resulted in significant and dose-dependent down-regulation of PI3K and Akt protein expressions.Video ClipWestern blot assay: 2 min 1 sec |
| Databáze: | OpenAIRE |
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