Simultaneous determination of five amino acid neurotransmitters in rat and porcine blood and brain by two-dimensional liquid chromatography
Autor: | Zhao-Ying Liu, Hui Yu, Sha-Sha Liu, Mo-Huan Tang, Ming-Jun Zhang, Xue-Jiao Zhao, Na Wang |
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Rok vydání: | 2020 |
Předmět: |
Analyte
Taurine Resolution (mass spectrometry) Swine Clinical Biochemistry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection Aspartic acid Animals Amino Acids Chromatography High Pressure Liquid gamma-Aminobutyric Acid chemistry.chemical_classification Brain Chemistry Neurotransmitter Agents Chromatography 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Glutamic acid 0104 chemical sciences Amino acid Rats chemistry Glycine Linear Models |
Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1163 |
ISSN: | 1873-376X |
Popis: | A method for the simultaneous determination of aspartic acid (Asp), glutamic acid (Glu), glycine (Gly), taurine (Tau) and gamma-aminobutyric acid (GABA) in animal blood and brain by two-dimensional liquid chromatography (2D-LC) combined with ultraviolet detection was established for the first time. First, the amino acid neurotransmitters (AANTs) were labeled on the corresponding fluorescent derivatives with 4-fluoro-7-nitrobenzofurazan (NBD-F), enriched on the extraction column and automatically transferred to the analytical column to achieve on-line extraction and complete separation of the target components. This method exhibited good selectivity, and the correlation coefficients for the analyte calibration curves of were > 0.99. The intra- and inter-day precisions were ≤ 16.03, and the accuracies were in the range of 70.59–116.20%. The system realizes the rapid detection and stability quantification of the five AANTs, which proves that the alternative dilution method is feasible. The results show that the system has high loading capacity, excellent resolution, and good peak shape and is not affected by other endogenous substances. Moreover, the developed method has been successfully applied to the analysis of biological samples in the blood and whole brain of rats and pigs. The content of AANTs in the hippocampus and cortex of rats was higher than that in those of pigs. This method is expected to provide applicability for the determination of AANTs in pharmacological, pharmaceutical and clinical research in nervous science. |
Databáze: | OpenAIRE |
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