Cell cycle-related variation in subcellular localization of eIF3e/INT6 in human fibroblasts
| Autor: | C. J. Norbury, S. J. Watkins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Protein subunit
Eukaryotic Initiation Factor-3 Fibrosarcoma Active Transport Cell Nucleus Fluorescent Antibody Technique Biology Initiation factor Humans COP9 signalosome Nuclear export signal Cellular compartment Cells Cultured Cell Nucleus Cell Cycle Cell Biology General Medicine Original Articles Cell cycle Fibroblasts Subcellular localization Molecular biology Cell biology Cell Compartmentation Protein Subunits Nuclear localization sequence Signal Transduction |
| Zdroj: | Cell proliferation. 37(2) |
| ISSN: | 1365-2184 0960-7722 |
| Popis: | The Int‐6 gene is a site of mouse mammary tumour virus (MMTV) integration in murine tumours and INT6 protein has been identified independently as a subunit (eIF3e) of the eukaryotic translation initiation factor eIF3. In addition, the protein can interact with two other multi‐subunit complexes: the COP9 signalosome (CSN) and the proteasome. The role of INT6 in tumourigenesis is nonetheless currently unclear. Here, using immunofluorescence microscopy, we show that eIF3e/INT6 is localized in part to the nucleus, while other eIF3 components are cytoplasmic. Primary human fibroblasts, but not their transformed counterparts, showed reduced nuclear INT6 staining in some cells, and this reduction was maximal in early S phase. This variation in eIF3e/INT6 may indicate regulated shuttling between cellular compartments and would be consistent with the presence of a nuclear export signal as well as a nuclear localization signal in the protein sequence. Loss of regulation of eIF3e/INT6 redistribution may therefore be a significant feature of malignancy in human cells. |
| Databáze: | OpenAIRE |
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