Antibiotic resistance and molecular typing among cockle (Anadara granosa) strains of Vibrio parahaemolyticus by polymerase chain reaction (PCR)-based analysis
| Autor: | A. Aminah, H. Osman, H. Mohd. Sallehuddin, R. A. S. Laila, A. M. Sahilah, A A Azuhairi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Genotype
Physiology Ceftazidime Microbial Sensitivity Tests Bacitracin Biology Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology law.invention law Ampicillin Drug Resistance Bacterial medicine Animals Cluster Analysis Cardiidae Polymerase chain reaction Vibrio parahaemolyticus Malaysia food and beverages General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Virology Anti-Bacterial Agents Random Amplified Polymorphic DNA Technique RAPD Molecular Typing Genes Bacterial Streptomycin Amikacin Biotechnology medicine.drug |
| Zdroj: | World Journal of Microbiology and Biotechnology. 30:649-659 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-013-1494-y |
| Popis: | Genomic DNA of Vibrio parahaemolyticus were characterized by antibiotic resistance, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. These isolates originated from 3 distantly locations of Selangor, Negeri Sembilan and Melaka (East coastal areas), Malaysia. A total of 44 (n = 44) of tentatively V. parahaemolyticus were also examined for the presence of toxR, tdh and trh gene. Of 44 isolates, 37 were positive towards toxR gene; while, none were positive to tdh and trh gene. Antibiotic resistance analysis showed the V. parahaemolyticus isolates were highly resistant to bacitracin (92 %, 34/37) and penicillin (89 %, 33/37) followed by resistance towards ampicillin (68 %, 25/37), cefuroxime (38 %, 14/37), amikacin (6 %, 2/37) and ceftazidime (14 %, 5/37). None of the V. parahaemolyticus isolates were resistant towards chloramphenicol, ciprofloxacin, ceftriaxone, enrofloxacin, norfloxacin, streptomycin and vancomycin. Antibiogram patterns exhibited, 9 patterns and phenotypically less heterogenous when compared to PCR-based techniques using ERIC- and RAPD-PCR. The results of the ERIC- and RAPD-PCR were analyzed using GelCompare software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the V. parahaemolyticus isolates into 6 clusters and 21 single isolates at a similarity level of 80 %. While, RAPD-PCR with primer Gen8 discriminated the V. parahaemolyticus isolates into 11 clusters and 10 single isolates and Gen9 into 8 clusters and 16 single isolates at the same similarity level examined. Results in the presence study demonstrated combination of phenotypically and genotypically methods show a wide heterogeneity among cockle isolates of V. parahaemolyticus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink