Hydrocephalus induced via intraventricular kaolin injection in adult rats
Autor: | Wang Zhanxiang, Chen Donghan, Shen Shanghang, Bao Jianfeng, Huang Caiquan, Zhang Feifei, Xu Hao, Zhang Shaolin, Liu Feng |
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Rok vydání: | 2015 |
Předmět: |
Male
Adult male Saline injection Morris water navigation task Pathology and Forensic Medicine Rats Sprague-Dawley Animal model hemic and lymphatic diseases medicine Animals Kaolin Maze Learning Injections Intraventricular medicine.diagnostic_test business.industry Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging Hydrocephalus Rats medicine.anatomical_structure Ventricle Anesthesia Spatial learning Neurology (clinical) business |
Zdroj: | Folia neuropathologica. 53(1) |
ISSN: | 1509-572X |
Popis: | Hydrocephalus is a common neurological disease in humans, but a uniform and particularly effective hydrocephalic animal model amenable to proper appraisal and deep study has not yet been established. In this study, we attempted to construct a high-efficiency model of hydrocephalus via intraventricular kaolin injection. Adult male Sprague-Dawley rats were randomly divided into 2 groups: the control group (n = 15) and the experimental group (n = 30). Kaolin was injected into the lateral ventricle of experimental animals. Control rats underwent the same procedure but received sterile saline injection instead of kaolin. All animals with kaolin injection into the lateral ventricle developed hydrocephalus according to magnetic resonance imaging (MRI) results (success rate up to 100%). Also, the Morris water maze (MWM) test demonstrated disturbed spatial learning and memory. Furthermore, there were significant differences between groups with respect to the histological changes in the periventricular tissue. Our results indicate that experimental hydrocephalus induced by lateral ventricle injection of kaolin in adult rats is feasible and may be widely used. |
Databáze: | OpenAIRE |
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