Development and validation an HPLC - UV method for determination of esomeprazole and pirfenidone simultaneously in rat plasma: application to a drug monitoring study
| Autor: | Sema T. Koz, Emrah Dural, Suleyman Koz |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Drug
HPLC-UV Chromatography Farmakoloji ve Eczacılık Chemistry Rat Plasma media_common.quotation_subject Phosphate buffered saline Esomeprazole Pirfenidone Esomeprazole pirfenidone rat plasma method validation HPLC-UV Method Validation Combined treatment Health Care Sciences and Services medicine Sağlık Bilimleri ve Hizmetleri Pharmacology and Pharmacy media_common medicine.drug |
| Zdroj: | Volume: 51, Issue: 1 16-25 İstanbul Journal of Pharmacy |
| ISSN: | 2587-2087 |
| DOI: | 10.26650/istanbuljpharm.2020.0091 |
| Popis: | Background and Aims: It has been observed that the combined treatment of esomeprazole and pirfenidone provides increased efficacy in the treatment of pulmonary fibrosis disease, recently. The aim of this study is to develop a simple, sensitive, and reliable high-performance liquid chromatography method to be used in drug monitoring to increase the effectiveness of esomeprazole and pirfenidone in treatment and to reduce their adverse effects. Methods: Separation was conducted with a C18 reverse-phase column (4.6 mm x 250 mm, 5 mu m) used as a mobile phase prepared with the phosphate buffer (10 mM KH2PO4 and 10 mM K2HPO4) and acetonitrile (60:40, v/v) by an isocratic flow (1 mL/min). Mobile phase pH was adjusted to 3.0. Ultraviolet detection was accomplished at 305 nm. The column oven was held at 35 degrees C to ensure an efficient analytical separation. Results: Analytical recovery of esomeprazole was between 92.43 and 105.36% and for pirfenidone it was found between 89.56 and 104.32%. Accuracy values of esomeprazole and pirfenidone were determined between (-2.90) - 4.22 and (-4.45) - 5.78, respectively. Precision (RSD%) was |
| Databáze: | OpenAIRE |
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