Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells
| Autor: | Sofien Ben Ammou, Anne Vejux, Meryam Debbabi, Stéphane Grégoire, Philippe Durand, Nadia Mekahli, Emmanuelle Camus, Lionel Bretillon, Amira Zarrouk, Maryem Bezine, Lucy Martine, Aymen Jabrane, Gérard Lizard, Mohamed Hammami, Thibault Moreau, Randa Sghaier, Michel Prost, Thomas Nury |
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| Přispěvatelé: | Lizard, Gérard, Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique (Bio-PeroxIL), Université de Bourgogne (UB), Université de Monastir - University of Monastir (UM), Faculté de médecine de Sousse [Ibn EL Jazzar], Université Tunis El Manar (UTM), Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), OEil, nutrition et signalisation cellulaire [CSGA], Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche Appliquée Spiral [Bourgogne] (LARA SPIRAL), Service de Neurologie générale, vasculaire et dégénérative (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Hôpital Universitaire Sahloul (CHU Sahloul), Université de Bourgogne (Dijon, France), Université de Monastir (Monastir, Tunisia), Université El Manar (Tunis, Tunisia), ASSAD (Louhans, France), Nopal Nutra (Dijon, France), and Departments of Neurology (University Hospital, Dijon, France) and (University Hospital, Sousse, Tunisia)., Université de Tunis El Manar (UTM), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Laboratoire Bio-PeroxIL. Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique ( Bio-PeroxIL ), Université de Bourgogne ( UB ), Nutrition, Aliments Fonctionnels et Santé Vasculaire, Université de Monastir ( UM ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Recherche Appliquée Spiral [Bourgogne] ( LARA SPIRAL ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), University Hospital of Sahloul |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant Organes des sens medicine.medical_treatment Trolox [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology alpha-Tocopherol MESH: Antioxidants/pharmacology MESH: Peroxisomes/drug effects Antioxidants biochimie métabolique lcsh:Chemistry Lipid peroxidation Mice chemistry.chemical_compound peroxisome MESH: Animals lcsh:QH301-705.5 Ketocholesterols Spectroscopy Membrane Potential Mitochondrial α-tocopherol γ-tocopherol oleic acid mitochondria murine microglial BV-2 cells peroxydation lipidique Neurodegeneration General Medicine Peroxisome 3. Good health Computer Science Applications MESH: Membrane Potential Mitochondrial/drug effects Biochemistry [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Microglia/metabolism Alimentation et Nutrition Microglia MESH: Mitochondria/pathology MESH: Mitochondria/drug effects acide oléique Programmed cell death MESH: gamma-Tocopherol/pharmacology Sensory Organs Médecine humaine et pathologie Biology Article Catalysis Cell Line Inorganic Chemistry 03 medical and health sciences MESH: Oleic Acid/pharmacology Peroxisomes medicine Animals Food and Nutrition Propidium iodide Physical and Theoretical Chemistry Olive Oil Molecular Biology MESH: Mice MESH: Peroxisomes/pathology maladie neurodégénérative MESH: Microglia/drug effects gamma-Tocopherol MESH: Ketocholesterols/pharmacology Organic Chemistry Neurosciences gène mitochondrial medicine.disease MESH: Olive Oil/pharmacology MESH: Cell Line Oleic acid 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Neurons and Cognition Human health and pathology MESH: alpha-Tocopherol/pharmacology |
| Zdroj: | International Journal of Molecular Sciences; Volume 17; Issue 12; Pages: 1973 International Journal of Molecular Sciences 12 (17), 1973. (2016) International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2016, 17 (12), pp.E1973. ⟨10.3390/ijms17121973⟩ International Journal of Molecular Sciences, Vol 17, Iss 12, p 1973 (2016) International Journal of Molecular Sciences, MDPI, 2016, 17 (12), pp.E1973. 〈http://www.mdpi.com/1422-0067/17/12/1973〉. 〈10.3390/ijms17121973〉 |
| ISSN: | 1422-0067 1661-6596 |
| DOI: | 10.3390/ijms17121973 |
| Popis: | Lipid peroxidation products, such as 7-ketocholesterol (7KC), may be increased in the body fluids and tissues of patients with neurodegenerative diseases and trigger microglial dysfunction involved in neurodegeneration. It is therefore important to identify synthetic and natural molecules able to impair the toxic effects of 7KC. We determined the impact of 7KC on murine microglial BV-2 cells, especially its ability to trigger mitochondrial and peroxisomal dysfunction, and evaluated the protective effects of alpha- and gamma-tocopherol, Trolox, and oleic acid (OA). Multiple complementary chemical assays, flow cytometric and biochemical methods were used to evaluate the antioxidant and cytoprotective properties of these molecules. According to various complementary assays to estimate antioxidant activity, only alpha-, and gamma-tocopherol, and Trolox had antioxidant properties. However, only alpha-tocopherol, gamma-tocopherol and OA were able to impair 7KC-induced loss of mitochondrial transmembrane potential, which is associated with increased permeability to propidium iodide, an indicator of cell death. In addition, alpha-and gamma-tocopherol, and OA were able to prevent the decrease in Abcd3 protein levels, which allows the measurement of peroxisomal mass, and in mRNA levels of Abcd1 and Abcd2, which encode for two transporters involved in peroxisomal beta-oxidation. Thus, 7KC-induced side effects are associated with mitochondrial and peroxisomal dysfunction which can be inversed by natural compounds, thus supporting the hypothesis that the composition of the diet can act on the function of organelles involved in neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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