Detection of Mycoplasma hyopneumoniae in Lung and Nasal Swab Samples from Pigs by Nested PCR and Culture Methods
| Autor: | Shigeto Yazawa, Tetsuo Asai, Isao Shibata, Yukiko Otagiri, Hidetoshi Hirai, Shizuo Sato, Takehiko Uto, Munenori Okada |
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| Rok vydání: | 2005 |
| Předmět: |
Nasal cavity
Lung General Veterinary biology Sus scrofa Mycoplasma hyopneumoniae Pneumonia of Swine Mycoplasmal respiratory system biology.organism_classification Polymerase Chain Reaction Virology Microbiology law.invention Titer medicine.anatomical_structure law Nasal Swab medicine Herd Animals Nasal Cavity Nested polymerase chain reaction Polymerase chain reaction |
| Zdroj: | Journal of Veterinary Medical Science. 67:801-805 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.67.801 |
| Popis: | We examined nasal swab and lung homogenate samples collected from pigs experimentally and naturally infected with Mycoplasma hyopneumoniae for the detection of M. hyopneumoniae by the nested PCR (nPCR) and culture methods. In the 23 experimentally infected pigs, M. hyopneumoniae was commonly detected in nasal swabs by the nPCR and culture methods at 4 weeks after inoculation, and there was a significant correlation (P |
| Databáze: | OpenAIRE |
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