MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
| Autor: | Yi Zhang, Jianjun Wang, Hongling Su |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular proliferation Biology medicine.disease_cause migration Real-Time Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor medicine Humans Luciferase Genes Tumor Suppressor Original Research Cell Proliferation Cell growth Liver Neoplasms Membrane Proteins miR-3150b General Medicine Golgi phosphoprotein 3 hepatocellular carcinoma medicine.disease digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Golgi Phosphoprotein 3 Carcinogenesis Wound healing |
| Zdroj: | Journal of Investigative Medicine |
| ISSN: | 1708-8267 1081-5589 |
| Popis: | Background In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis. Methods The expression of miR-3150b and Golgi phosphoprotein 3 (GOLPH3) was determined in HCC cell lines. Cell proliferation, migration and invasion were estimated by Cell Counting Kit-8, wound healing and Transwell assays. The association between miR-3150b and GOLPH3 was verified by luciferase assay. Results MiR-3150b was downregulated, while GOLPH3 was remarkably upregulated in HCC cells. Furthermore, miR-3150b inhibited HCC cell proliferation, migration and invasion. MiR-3150b directly targeted and negatively regulated GOLPH3. Conclusion MiR-3150b suppressed HCC cell proliferation, invasion and migration by targeting GOLPH3. |
| Databáze: | OpenAIRE |
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