Isolation and identification of proteolytic lactic-acid bacteria of the common carp (Cyprinus carpio) by spontaneous fermentation to obtain functional peptides

Autor: María Luisa, Sahagún-Aguilar, Socorro Josefina, Villanueva-Rodríguez, Gabriel, Rincón-Enríquez, Manuel Reinhart, Kirchmayr, Eugenia, Del Carmen Lugo-Cervantes, María Dolores, García-Parra
Rok vydání: 2022
Předmět:
Zdroj: Braz J Microbiol
ISSN: 1678-4405
1517-8382
Popis: High proteolytic activity and several biological functions (antimicrobial, antioxidant, antihypertensive, among others) have been attributed to lactic-acid bacteria (LAB) isolated from fish and peptides obtained from proteolysis. Therefore, the objective of this research was isolating, characterizing, and identifying LAB with proteolytic activity by spontaneous fermentation from common carp (Cyprinus carpio) reared in ponds and wild ones obtained from Lago de Chapala, Jalisco, Mexico. Spontaneous fermentation from complete carp specimens was observed, considering two sampling points (skin and intestines) at 15 °C at 5 and 10 days. Isolated LAB—from both reared and wild specimens—were identified and morphologically characterized; identification was performed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Proteolytic activity was assessed by the presence of the proteolytic halo. A total of five genera and eight different LAB proteolytic species were isolated from all the carp samples. At 10 days, greater proteolytic LAB diversity was obtained from the intestine (Tukey’s, p
Databáze: OpenAIRE
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