Abnormal extracellular matrix remodelling in the cervix of pregnant relaxin-deficient mice is not associated with reduced matrix metalloproteinase expression or activity
| Autor: | Yu May Soh, Laura J. Parry, Emma Simpson, Helen M. Gehring, Jonathan T. McGuane, Sarah A. Marshall |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty MMP2 Receptors Peptide Reproductive technology Cervix Uteri Biology MMP9 MMP7 Receptors G-Protein-Coupled Extracellular matrix 03 medical and health sciences Mice Endocrinology Pregnancy Internal medicine Genetics medicine Gelatinase Animals Molecular Biology Relaxin Mice Knockout Matrix Metalloproteinases Extracellular Matrix body regions 030104 developmental biology Reproductive Medicine Gelatinases Animal Science and Zoology Female Developmental Biology Biotechnology Relaxin receptor |
| Zdroj: | Reproduction, fertility, and development. 30(9) |
| ISSN: | 1031-3613 |
| Popis: | Relaxin regulates cervical extracellular matrix (ECM) remodelling during pregnancy by modifying collagen and other ECM molecules by unknown mechanisms. We hypothesised that abnormal collagen remodelling in the cervix of pregnant relaxin-deficient (Rln1−/−) mice is due to excessive collagen (Col1a1 and Col3a1) and decreased matrix metalloproteinases (Mmp2, Mmp9, Mmp13 and Mmp7) and oestrogen receptors (Esr1 and Esr2). Quantitative polymerase chain reaction, gelatinase zymography, MMP activity assays and histological staining evaluated changes in ECM in pregnant wildtype (Rln1+/+) and Rln1−/− mice. Cervical Col1a1, Col3a1 and total collagen increased in Rln1−/− mice and were higher at term compared with Rln1+/+ mice. This was not correlated with a decrease in gelatinase (Mmp2, Mmp9) expression or activity, Mmp7 or Mmp13 expression, which were all significantly higher in Rln1−/− mice. In late pregnancy, circulating MMP2 and MMP9 were unchanged. Esr1 expression was highest in Rln1+/+ and Rln1−/− mice in late pregnancy, coinciding with a decrease in Esr2 in Rln1+/+ but not Rln1−/− mice. The relaxin receptor (Rxfp1) decreased slightly in late-pregnant Rln1+/+ mice, but was significantly higher in Rln1−/− mice. In summary, relaxin deficiency results in increased cervical collagen in late pregnancy, which is not explained by a reduction in Mmp expression or activity or decreased Rxfp1. However, an imbalance between Esr1 and Esr2 may be involved. |
| Databáze: | OpenAIRE |
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