Indocyanine Green-001 (ICG-001) Attenuates Wnt/β-catenin-induces Myocardial Injury Following Sepsis
| Autor: | Alaa Manea Hassan, Nasser Ghaly Yousif, Najah R. Hadi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty H&E stain 030204 cardiovascular system & hematology Sepsis sepsis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Medicine Pharmacology (medical) myocardial injury Pharmacology business.industry Monocyte Wnt signaling pathway Indocyanine green-100 medicine.disease 030104 developmental biology medicine.anatomical_structure chemistry Ventricle Catenin business Indocyanine green Research Paper |
| Zdroj: | Journal of Pharmacology & Pharmacotherapeutics |
| ISSN: | 0976-5018 0976-500X |
| Popis: | Objective: To investigate the mechanistic pathway of both indocyanine green (ICG)-001 in attenuated endotoxemia-induced cardiac depression through downregulation cardiac Wnt/ β-catenin cell signaling. Materials and Methods: Adult (4–6 months) male Albino-Webster mice, their weights ranged from 25 to 30 g, were pretreated with ICG-001 i.p., following cecal ligation and puncture (CLP). Left ventricle (LV) function was assessed using a microcatheter system. Monocyte chemoattractant protein-1 (MCP-1) and cytokines mediators in plasma and myocardium were analyzed by enzyme-linked immunosorbent assay. Further, the cardiac Wnt protein measured by quantitative real-time polymerase chain reaction while β-catenin analysis through Western blotting procedure. The pathological changes and cells injury in the myocardium were examined using hematoxylin and eosin staining. Results: CLP mice displayed worse LV function. The exaggerated cardiac depression in CLP mice was associated with higher levels of MCP-1 and cytokines in plasma and myocardium together with greater cardiac levels of cardiac troponin-I and Wnt/β-catenin. Neutralization of sepsis by either ICG-001resulted in improved LV function and reductions in inflammatory mediators. Conclusion: Taken together, these data showed that ICG-001 improved LV function following sepsis through downregulation of Wnt/β-catenin and serve as a potential mechanistic pathway ICG-001 in therapeutic cardiac endotoxemia in animal model. |
| Databáze: | OpenAIRE |
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