Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe

Autor: Jerome Follet, Martin Kváč, Cláudia A. Ribeiro, Sébastien Détriché, A. Barbier Bourgeois, M. Dellevoet-Groenewegen, Sumaiya Hoque, Yvonne Daandels, Ourida Hammouma, Pedro Pinto, Evi Canniere, Hélène Leruste, Janine Roemen, Anastasios D. Tsaousis
Přispěvatelé: Faculdade de Engenharia da Universidade do Porto (FEUP), Universidade do Porto = University of Porto, Faculdade de Medicina da Universidade do Porto (FMUP), JUNIA (JUNIA), Université catholique de Lille (UCL), Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 (LGCgE), Université d'Artois (UA)-Université de Lille-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), This project has received funding from the Interreg 2 Seas programme 2014-2020 co-funded by the European Regional Development Fund under subsidy contract No 2S05-043., Universidade do Porto, Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Microbiology (medical)
Veterinary medicine
QH301-705.5
Range (biology)
animal diseases
030231 tropical medicine
prevalence
Cryptosporidium
Microbiology
Cryptosporidium species
030308 mycology & parasitology
law.invention
[SPI]Engineering Sciences [physics]
03 medical and health sciences
0302 clinical medicine
law
Virology
parasitic diseases
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Biology (General)
Genotyping
Dairy cattle
2. Zero hunger
0303 health sciences
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
biology
business.industry
Incidence (epidemiology)
microbiology
dairy cattle
gp60
biology.organism_classification
Subtyping
QR
18S rRNA
Geography
Transmission (mechanics)
genotyping
Mainland
Livestock
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
business
Zdroj: Microorganisms
Microorganisms, 2021, 9 (11), pp.2394. ⟨10.3390/microorganisms9112394⟩
Volume 9
Issue 11
Microorganisms, Vol 9, Iss 2394, p 2394 (2021)
Microorganisms, MDPI, 2021, 9 (11), pp.2394. ⟨10.3390/microorganisms9112394⟩
ISSN: 2076-2607
DOI: 10.3390/microorganisms9112394⟩
Popis: Cryptosporidium is comprised an apicomplexan parasitic protist, which infects a wide range of hosts, causing cryptosporidiosis. In cattle farms, the incidence of cryptosporidiosis results in high mortality in calves leading to considerable economic loss in the livestock industry. Infected animals may also act as a major reservoir of Cryptosporidium spp., in particular C. parvum, the most common cause of cryptosporidiosis in calves. This poses a significant risk to other farms via breeding centres, to trading of livestock and to human health. This study, funded by the Interreg-2-seas programme, is a part of a global project aimed at strategies to tackle cryptosporidiosis. To reach this target, it was essential to determine whether prevalence was dependent on the studied countries or if the issue was borderless. Indeed, C. parvum occurrence was assessed across dairy farms in certain regions of Belgium, France and the Netherlands. At the same time, the animal-to-animal transmission of the circulating C. parvum subtypes was studied. To accomplish this, 1084 faecal samples, corresponding to 57 dairy-farms from all three countries, were analysed. Well-established protocols amplifying the 18S rDNA and gp60 genes fragments, followed by DNA sequencing, were used for the detection and subtyping C. parvum; the DNA sequences obtained were further characterised using a combination of bioinformatics and phylogenetics methods. Our results show 25.7%, 24.9% and 20.8% prevalence of Cryptosporidium spp. in Belgium, France and the Netherlands respectively. Overall, 93% of the farms were Cryptosporidium positive. The gp60 subtyping demonstrated a significant number of the C. parvum positives belonged to the IIa allelic family, which has been also detected in humans. Consequently, this study highlights how widespread is C. parvum in dairy farms and endorses cattle as a major carrier of zoonotic C. parvum subtypes, which subsequently pose a significant threat to human health.
Databáze: OpenAIRE
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