Relationships between in vivo and in vitro aflatoxin production: reliable prediction of fungal ability to contaminate maize with aflatoxins
| Autor: | Claudia Probst, Peter J. Cotty |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Aflatoxin
Biological pest control Aspergillus flavus Mycology Biology Zea mays chemistry.chemical_compound Aflatoxins In vivo Genetics heterocyclic compounds Mycotoxin Ecology Evolution Behavior and Systematics business.industry food and beverages Contamination biology.organism_classification In vitro Culture Media Biotechnology Infectious Diseases chemistry Fermentation business |
| Zdroj: | Fungal Biology. 116:503-510 |
| ISSN: | 1878-6146 |
| Popis: | Aflatoxins are highly carcinogenic mycotoxins frequently produced by Aspergillus flavus. Contamination of maize with aflatoxins imposes both economic and health burdens in many regions. Identification of the most important etiologic agents of contamination is complicated by mixed infections and varying aflatoxin-producing potential of fungal spe- cies and individuals. In order to know the potential importance of an isolate to cause a con- tamination event, the ability of the isolate to produce aflatoxins on the living host must be determined. Aflatoxin production in vitro (synthetic and natural media) was contrasted with in vivo (viable maize kernels) in order to determine ability of in vitro techniques to pre- dict the relative importance of causal agents to maize contamination events. Several media types and fermentation techniques (aerated, non-aerated, fermentation volume) were compared. There was no correlation between aflatoxin production in viable maize and pro- duction in any of the tested liquid fermentation media using any of the fermentation tech- niques. Isolates that produced aflatoxins on viable maize frequently failed to produce detectable (limit of detection ¼ 1 ppb) aflatoxin concentrations in synthetic media. Aflatoxin production on autoclaved maize kernels was highly correlated with production on viable maize kernels. The results have important implications for researchers seeking to either identify causal agents of contamination events or characterize atoxigenic isolates for biological control. Published by Elsevier Ltd on behalf of The British Mycological Society. |
| Databáze: | OpenAIRE |
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