Mechanism of the Anti-Cancer Activity of Zizyphus jujuba in HepG2 Cells
| Autor: | Toshio Norikura, Xuedan Huang, David Opare Kennedy, Isao Matsui-Yuasa, Tadayoshi Hasuma, Akiko Kojima-Yuasa |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
G2 Phase
Cell cycle checkpoint Cell Survival Antineoplastic Agents Apoptosis Pharmacology Retinoblastoma Protein Cell Line Tumor medicine Humans Phosphorylation Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species Traditional medicine Chemistry G1 Phase Ziziphus General Medicine Cell cycle Complementary and alternative medicine Mechanism of action Cell culture Solvents Chloroform medicine.symptom Reactive Oxygen Species Cyclin-Dependent Kinase Inhibitor p27 Intracellular Drugs Chinese Herbal |
| Zdroj: | The American Journal of Chinese Medicine. 35:517-532 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x0700503x |
| Popis: | The Zizyphus jujuba fruit has been used as a traditional Chinese medicinal herb and considered to affect various physiological functions in the body for thousands of years. However, its anti-cancer activity and mechanism of action remain to be elucidated. We investigated the anti-cancer activity of Zizyphus jujuba Mill and its underlining mechanisms of action in human hepatoma cells (HepG2) and found that the extract of Z. jujuba decreased the viability of the cells. Further extraction of the initial Z. jujuba extract with organic solvents revealed that the chloroform fraction ( CHCl 3-F) was the most effective. Interestingly, the CHCl 3-F induced not only apoptosis but also G1 arrest at a low concentration (100 μg/ml) and G2/M arrest at a higher concentration (200 μg/ml) by cell cycle assay. Apoptosis, an increase in intracellular ROS (reactive oxygen species) level, a decline of mitochondrial membrane potential at low Z. jujuba concentrations, and a ROS-independent mitochondrial dysfunction pathway at high concentrations were all observed. CHCl 3-F-induced G1 arrest in HepG2 cells was associated with an increase in hypohosphorylation of Rb and p27Kip1, and a decrease of phosphorylated Rb . However, CHCl 3-F-induced G2/M arrest in HepG2 cells correlated with a decrease of the p27Kip1 levels and generation of the phosphorylation of p27Kip1, however the hypohosphorylation of Rb protein remained. Collectively, our findings suggest that the CHCl 3-F extract of Z. jujuba extract induced a concentration dependent effect on apoptosis and a differential cell cycle arrest in HepG2 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|