Rye Snow Mold-Associated Microdochium nivale Strains Inhabiting a Common Area: Variability in Genetics, Morphotype, Extracellular Enzymatic Activities, and Virulence
| Autor: | M. L. Ponomareva, E. V. Osipova, Kim Potapov, S. A. Ponomarev, Natalia E. Gogoleva, Azat Meshcherov, Olga V. Petrova, Viktor Korzun, Gorshkov Vladimir A, Alexander S. Balkin, Olga Gogoleva, Yuri Gogolev, E. P. Vetchinkina |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Secale Genetics Microdochium plant–microbe interactions biology snow mold fungi Virulence food and beverages Plant Science biology.organism_classification Snow mold Plant disease virulence Microdochium nivale lcsh:Biology (General) Extracellular plant cell-wall-degrading enzymes Internal transcribed spacer Psychrophile human activities lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Fungi, Vol 6, Iss 335, p 335 (2020) Journal of Fungi Volume 6 Issue 4 |
| Popis: | Snow mold is a severe plant disease caused by psychrophilic or psychrotolerant fungi, of which Microdochium species are the most harmful. A clear understanding of Microdochium biology has many gaps the pathocomplex and its dynamic are poorly characterized, virulence factors are unknown, genome sequences are not available, and the criteria of plant snow mold resistance are not elucidated. Our study aimed to identify comprehensive characteristics of a local community of snow mold-causing Microdochium species colonizing a particular crop culture. By using the next-generation sequencing (NGS) technique, we characterized fungal and bacterial communities of pink snow mold-affected winter rye (Secale cereale) plants within a given geographical location shortly after snowmelt. Twenty-one strains of M. nivale were isolated, classified on the basis of internal transcribed spacer 2 (ITS2) region, and characterized by morphology, synthesis of extracellular enzymes, and virulence. Several types of extracellular enzymatic activities, the level of which had no correlations with the degree of virulence, were revealed for Microdochium species for the first time. Our study shows that genetically and phenotypically diverse M. nivale strains simultaneously colonize winter rye plants within a common area, and each strain is likely to utilize its own, unique strategy to cause the disease using &ldquo a personal&rdquo pattern of extracellular enzymes. |
| Databáze: | OpenAIRE |
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