Enzyme recovery during gas/liquid two-phase flow microfiltration of enzyme/yeast mixtures
| Autor: | C. Fonade, Muriel Mercier-Bonin |
|---|---|
| Přispěvatelé: | Unité mixte de recherche biotechnologies bioprocédés, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2002 |
| Předmět: |
Quality Control
0106 biological sciences Glycoside Hydrolases [SDV]Life Sciences [q-bio] Microfiltration Restriction Mapping Ultrafiltration Bioengineering Saccharomyces cerevisiae Sensitivity and Specificity 01 natural sciences 7. Clean energy Applied Microbiology and Biotechnology Permeability 03 medical and health sciences 010608 biotechnology Enzyme Stability [SDV.IDA]Life Sciences [q-bio]/Food engineering [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Colloids 030304 developmental biology 0303 health sciences Chromatography beta-Fructofuranosidase Chemistry Reproducibility of Results Membranes Artificial Permeation Slug flow Enzymes Volumetric flow rate Solutions Diafiltration Membrane Flow conditions Adsorption Gases Two-phase flow Powders Biotechnology |
| Zdroj: | Biotechnology and Bioengineering Biotechnology and Bioengineering, 2002, 80 (6), pp.610-621. ⟨10.1002/bit.10415⟩ Biotechnology and Bioengineering, Wiley, 2002, 80 (6), pp.610-621. ⟨10.1002/bit.10415⟩ |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.10415 |
| Popis: | The effect of a gas/liquid two-phase flow on the recovery of an enzyme was evaluated and compared with standard crossflow operation when confronted with the microfiltration of a high-fouling yeast suspension. Ceramic tubular and flat sheet membranes were used. At constant feed concentration (permeate recycling) and transmembrane pressure, the results obtained with the tubular membrane were dependent on the two-phase flow pattern. In comparison with single-phase flow performances at the same liquid velocity, the enzyme transmission was maintained at a high level with a bubble flow pattern but it decreased by 70% with a slug flow, whatever the flow rate ratio. Identical results were obtained with flat sheet membranes: for the highest flow rate ratio, the enzyme transmission was reduced by 70% even though the permeate flux was improved by 240%. During diafiltration experiments with the tubular membrane, it was found that a bubble flow pattern led to a 13% higher enzyme recovery compared to single-phase flow conditions, whereas with a slug flow the enzyme recovery was strongly reduced. With bubble flow conditions, energy consumption was minimal, confirming that this flow pattern was the most suitable for enzyme recovery. |
| Databáze: | OpenAIRE |
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