Shedding light on the expansion and diversification of the Cdc48 protein family during the rise of the eukaryotic cell
| Autor: | Dirk Fasshauer, Tobias H. Kloepper, Nickias Kienle |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein family Eukaryogenesis AAA+ proteins SELMA Valosin-containing protein Protein domain Cell Cycle Proteins Vesicle trafficking Evolution Molecular 03 medical and health sciences Protein Domains Valosin Containing Protein Phylogenetics LECA Organelle NSF Phylogeny Ecology Evolution Behavior and Systematics Organelles Adenosine Triphosphatases Genetics biology Compartmentalization (psychology) Biological Evolution Markov Chains AAA proteins Eukaryotic Cells 030104 developmental biology Compartmentalization Prokaryotic Cells Evolutionary biology biology.protein Function (biology) Research Article |
| Zdroj: | BMC Evolutionary Biology BMC evolutionary biology, vol. 16, no. 1, pp. 215 |
| ISSN: | 1471-2148 |
| DOI: | 10.1186/s12862-016-0790-1 |
| Popis: | Background A defining feature of eukaryotic cells is the presence of various distinct membrane-bound compartments with different metabolic roles. Material exchange between most compartments occurs via a sophisticated vesicle trafficking system. This intricate cellular architecture of eukaryotes appears to have emerged suddenly, about 2 billion years ago, from much less complex ancestors. How the eukaryotic cell acquired its internal complexity is poorly understood, partly because no prokaryotic precursors have been found for many key factors involved in compartmentalization. One exception is the Cdc48 protein family, which consists of several distinct classical ATPases associated with various cellular activities (AAA+) proteins with two consecutive AAA domains. Results Here, we have classified the Cdc48 family through iterative use of hidden Markov models and tree building. We found only one type, Cdc48, in prokaryotes, although a set of eight diverged members that function at distinct subcellular compartments were retrieved from eukaryotes and were probably present in the last eukaryotic common ancestor (LECA). Pronounced changes in sequence and domain structure during the radiation into the LECA set are delineated. Moreover, our analysis brings to light lineage-specific losses and duplications that often reflect important biological changes. Remarkably, we also found evidence for internal duplications within the LECA set that probably occurred during the rise of the eukaryotic cell. Conclusions Our analysis corroborates the idea that the diversification of the Cdc48 family is closely intertwined with the development of the compartments of the eukaryotic cell. Electronic supplementary material The online version of this article (doi:10.1186/s12862-016-0790-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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