| Autor: |
Suhair Sunoqrot, Amani Al-Hadid, Ahmad Manasrah, Ruba Khnouf, Lina Hasan Ibrahim |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
RSC Advances. 11:39582-39592 |
| ISSN: |
2046-2069 |
| DOI: |
10.1039/d1ra07467a |
| Popis: |
Glucose oxidase (GOx) is an enzyme with important industrial and biochemical applications, particularly in glucose detection. Here we leveraged the oxidative self-polymerization phenomenon of simple polyphenols (pyrogallol or catechol) in the presence of polyethylenimine (PEI) to form adhesive coatings that enabled GOx immobilization on conventional multi-well plates. Immobilization was verified and optimized by directly measuring GOx activity inside the coated wells. Our results showed that incorporating PEI in polyphenol coatings enhanced their enzyme immobilization efficiency, with pyrogallol (PG)-based coatings displaying the greatest enzyme activity. The immobilized enzyme maintained similar affinity to glucose compared to the free enzyme. GOx-immobilized PG/PEI-coated wells exhibited intermediate recycling ability but excellent resistance to urea as a denaturing agent compared to the free enzyme. GOx-immobilized 96-well plates allowed the construction of a linear glucose calibration curve upon adding glucose standards, with a detection limit of 0.4-112.6 mg dL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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