More evidence for a particle character of aminoacyl-transfer RNA synthetases isolated from rat liver
| Autor: | Hans Bloemendal, Claudia Vennegoor, A.L.H. Stols |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Glutamine
Size-exclusion chromatography Biology law.invention Amino Acyl-tRNA Synthetases Methionine Structural Biology law Leucine Centrifugation Density Gradient Animals Centrifugation Isoleucine Molecular Biology GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Chromatography Isoelectric focusing Lysine RNA Bio-Molecular Chemistry Chromatography Ion Exchange Rats Sedimentation coefficient Microscopy Electron Biochemistry Liver Sephadex Transfer RNA Hydroxyapatites Electron microscope Isoelectric Focusing |
| Zdroj: | Journal of Molecular Biology, 65, 375-378 Journal of Molecular Biology, 65, 2, pp. 375-378 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(72)90289-6 |
| Popis: | Glutaminyl-, isoleucyl-, leucyl-, lysyl- and methionyl-transfer RNA synthetase can be isolated from a postmicrosomal fraction from rat liver as a complex which resists separation in the following techniques: gel filtration on Sephadex G200, chromatography on DEAE-Sephadex A50, chromatography on DEAE-Sephadex A50, chromatography on hydroxyapatite, centrifugation in sucrose gradients and isoelectric focusing. The complex has a sedimentation coefficient of 18 s. In the electron microscope it appears as an organized structure of well-defined size and shape. These observations might provide additional evidence for the particle character of aminoacyl-transfer RNA synthetases. |
| Databáze: | OpenAIRE |
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