Analysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies
| Autor: | W Donachie, I. McConnell, Deborah J. Allen, J Hopkins, J Huntley, P. Bird, P Jones |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.drug_class
Enzyme-Linked Immunosorbent Assay Biology Immunoglobulin light chain Monoclonal antibody Antigen Antibody Specificity medicine Animals Immunoelectrophoresis Lymph node Cells Cultured B cell B-Lymphocytes Sheep General Veterinary Antibodies Monoclonal Flow Cytometry Molecular biology Isotype Immunoglobulin Isotypes B-1 cell medicine.anatomical_structure Immunoglobulin M Immunoglobulin G Immunology biology.protein Immunoglobulin Light Chains Lymph Nodes Antibody |
| Zdroj: | Research in Veterinary Science. 59:189-194 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(95)90000-4 |
| Popis: | Monoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect