MZF-1/Elk-1/PKCα is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma
| Autor: | Jen Hsiang Tsai, Hsin-Ling Yang, Jin Ming Hwang, Jer Yuh Liu, Je Chiuan Ye, Chia Jen Lee, Meen Woon Hsiao, Li-Sung Hsu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MZF-1 business.industry PKCα Myeloid zinc finger 1 Alpha (ethology) Transfection medicine.disease Small hairpin RNA 03 medical and health sciences Elk-1 030104 developmental biology Oncology Hepatocellular carcinoma Cancer research Medicine HCC business Transcription factor Protein kinase C Research Paper Binding domain |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.20467 |
| Popis: | Background: Protein kinase C alpha (PKCα) is a key signaling molecule in human cancer development. As a therapeutic strategy, targeting PKCα is difficult because the molecule is ubiquitously expressed in non-malignant cells. PKCα is regulated by the cooperative interaction of the transcription factors myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) in human cancer cells. Methods: By conducting tissue array analysis, herein, we determined the protein expression of MZF-1/Elk-1/PKCα in various cancers. Results: The data show that the expression of MZF-1/Elk-1 is correlated with that of PKCα in hepatocellular carcinoma (HCC), but not in bladder and lung cancers. In addition, the PKCα down-regulation by shRNA Elk-1 was only observed in the HCC SK-Hep-1 cells. Blocking the interaction between MZF-1 and Elk-1 through the transfection of their binding domain MZF-160-72 decreased PKCα expression. This step ultimately depressed the epithelial-mesenchymal transition potential of the HCC cells. Conclusion: These findings could be used to develop an alternative therapeutic strategy against patients with the PKCα-derived HCC. |
| Databáze: | OpenAIRE |
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