Thyroid peroxidase glycosylation: The location and nature of the N-linked oligosaccharide units in porcine thyroid peroxidase
| Autor: | Gail Pollock, Allen B. Rawitch, Alvin Taurog, Shi-Xin Yang |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Glycosylation
Swine Molecular Sequence Data Carbohydrates Thyroid Gland Biophysics Oligosaccharides Mannose Biology Iodide Peroxidase Biochemistry chemistry.chemical_compound Thyroid peroxidase medicine Animals Trypsin Amino Acid Sequence Asparagine Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Glycopeptides Oligosaccharide Peptide Fragments chemistry biology.protein Glycoprotein medicine.drug Peroxidase |
| Zdroj: | Archives of Biochemistry and Biophysics. 297:321-327 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(92)90679-q |
| Popis: | Highly purified, trypsin/detergent-solubilized thyroid peroxidase (TPO), prepared from pig thyroid tissue, was subjected to reduction and alkylation followed by trypsin digestion. The resulting peptides were fractionated using HPLC. Corresponding carbohydrate positive regions from three separate HPLC experiments were pooled and further chromatography was carried out to yield purified peptide suitable for sequence analysis and complete carbohydrate composition analysis. Four of the five putative sites for N-linked glycosylation were found to carry oligosaccharide units in which mannose and glucosamine were the sole or predominant sugars. Three of the four glycosylations occur at asparagine residues which are likely to be at beta turns or bends. The fifth putative glycosylation site could not be confirmed and may either be poorly glycosylated or escape glycosylation. All of the confirmed glycosylated sites occur in the N-terminal third of the TPO polypeptide chain, in the portion of the molecule believed to be extracellular. The isolation of at least two chromatographic forms of glycopeptide derived from each of the confirmed sites suggests microheterogeneity in the structure of the oligosaccharide units of thyroid peroxidase similar to that observed in many other glycoproteins. |
| Databáze: | OpenAIRE |
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