Absence of TGF-β Receptor Activation by Highly Purified hCG Preparations
| Autor: | Katri Koli, Hannu Koistinen, Ulf-Håkan Stenman, Laura Hautala |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.medical_treatment Biology Chorionic Gonadotropin Human chorionic gonadotropin 03 medical and health sciences 0302 clinical medicine Endocrinology Pregnancy Epidermal growth factor Cell Line Tumor Internal medicine medicine Humans Receptor Molecular Biology reproductive and urinary physiology Original Research 030304 developmental biology 0303 health sciences urogenital system Growth factor Trophoblast General Medicine Transfection 3. Good health medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Female Receptors Transforming Growth Factor beta hormones hormone substitutes and hormone antagonists Signal Transduction Transforming growth factor |
| Zdroj: | Molecular Endocrinology. 29:1787-1791 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | Recently, several LH/human chorionic gonadotropin (hCG) receptor-independent activities for hCG have been described, including activation of the TGF-β receptor (TGFβR) by hyperglycosylated hCG and stimulation of trophoblast invasion. Because the hCG concentrations used in these studies have been rather high, reflecting physiological hCG levels in pregnancy, even a minor contamination with growth factors, which act at very low concentrations, may be significant. Several commercial hCG preparations have been found to contain significant amounts of epidermal growth factor (EGF), which we also confirmed here. Furthermore, we found that some hCG preparations also contain significant amounts of TGF-β1. These hCG preparations were able to activate ERK1/2 in JEG-3 choriocarcinoma cells or TGFβR in mink lung epithelial cells transfected with a reporter gene for TGFβR activation. No such activation was found with highly purified hCG or its free β-subunit (hCGβ), irrespective of whether they were hyperglycosylated or not. Taken together, our results suggest that the growth factor contaminations in the hCG preparations can cause activation of TGFβR and, at least in JEG-3 cells, MAPK signaling. This highlights the importance to carefully control for potential contaminations and that highly purified hCG preparations have to be used for biological studies. |
| Databáze: | OpenAIRE |
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