Spatial and temporal variations of the bacterial community in the bovine digestive tract
| Autor: | Jérôme Hamelin, Valérie Monteils, Thierry Gidenne, Laurent Cauquil, R. J. Michelland, Laurence Fortun-Lamothe, Sylvie Combes, Asma Zened |
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| Přispěvatelé: | Tissus animaux, nutrition, digestion, écosystème et métabolisme (TANDEM), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT], Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Rumen Microorganism Zoology Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Feces 03 medical and health sciences Microbial ecology RNA Ribosomal 16S Benzene Derivatives medicine Animals Large intestine CE-SSCP Polymorphism Single-Stranded Conformational BACTERIES PARAMETRES ENVIRONNEMENTAUX 030304 developmental biology 2. Zero hunger 0303 health sciences Bacteria biology 030306 microbiology Community structure Electrophoresis Capillary Biodiversity General Medicine biology.organism_classification Gastrointestinal Tract [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology medicine.anatomical_structure Cattle Propionates Reticulum Biotechnology |
| Zdroj: | Journal of Applied Microbiology Journal of Applied Microbiology, Wiley, 2009, pp.1642-1650. ⟨10.1111/j.1365-2672.2009.04346.x⟩ |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/j.1365-2672.2009.04346.x |
| Popis: | Aims: Improved knowledge of the bacterial community of the digestive tract is required to enhance the efficiency of digestion in herbivores. This work aimed to study spatial and temporal variations of the bacterial communities in the bovine digestive tract and their correlation with gut environmental parameters. Methods and Results: Rumen content and faeces of five cows were sampled for 3 weeks. In addition, reticulum content was sampled during the third week. Bacterial communities were assessed by studying capillary electrophoresis single-stranded conformation polymorphism (CE-SSCP) profiles of 16S rRNA genes. The bacterial community structure differed between the forestomach and faecal contents. The abundance of several operational taxonomic units changed from week to week. Bacterial community structure of the rumen was correlated to propionic acid and NH3–N concentrations. Conclusions: The bacterial community of the bovine digestive tract varied in space and time. Significance and Impact of the Study: The study of the bacterial communities of the digestive tract in herbivores should be widened from the rumen to the large intestine. The amplitude and origin of the temporal variation of the ruminal bacterial community need to be better understood to improve the control of the fermentative activity in herbivores. |
| Databáze: | OpenAIRE |
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