Endothelium on the flow surface of human aortic Dacron vascular grafts
| Autor: | Qun Shi, Moses Hong-De Wu, Yoko Onuki, Kaj Johansen, Lester R. Sauvage, Glenn C. Hunter, Rafik Ghali |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Endothelium Surface Properties Immunocytochemistry Pannus Giant Cells Specimen Handling Lectins medicine.artery von Willebrand Factor medicine Humans Coloring Agents Aorta Aged Factor VIII Polyethylene Terephthalates business.industry Microcirculation Capsule Anatomy Fibroblasts medicine.disease Immunohistochemistry Blood Vessel Prosthesis Staining Microscopy Electron medicine.anatomical_structure Connective Tissue Regional Blood Flow Giant cell Microscopy Electron Scanning Surgery Collagen Endothelium Vascular Tissue Preservation Implant Plant Lectins Cardiology and Cardiovascular Medicine business Aortic Aneurysm Abdominal |
| Zdroj: | Journal of Vascular Surgery. 25:736-742 |
| ISSN: | 0741-5214 |
| DOI: | 10.1016/s0741-5214(97)70303-3 |
| Popis: | Purpose: We have previously observed endothelium on two human vascular prostheses explanted under optimal conditions for flow surface preservation. In this study we sought to further verify the hypothesis that endothelialization can occur on clinical grafts and that it can be detected in specimens that have been promptly removed and properly preserved in a timely manner. Methods: We studied 29 aortic grafts. Of these, 11 Dacron bypass grafts were in a condition suitable for analysis with light microscopy and immunocytochemistry staining, and scanning and transmission electron microscopy. Results: Three grafts had endothelium beyond the pannus, identified by factor VIII/vWF, Ulex europaeus agglutinin, and collagen IV positivity. Specimen A, a knitted 6-year implant, was preserved by embalming 3 hours after the patient's death and had a firmly attached outer capsule with fibroblasts, collagen, giant cells, and microvessels in the interstices. Specimen B, a woven 18-year implant, was retrieved at reoperation and immediately fixed in 10% formalin; it had no outer capsule and no tissue ingrowth. Specimen C, a woven 7-year implant, was removed and fixed 5 hours after the patient's death; it had a firmly attached outer capsule but no tissue ingrowth beyond the outer portion of the wall. Conclusions: The rapidity with which the specimens were fixed probably enabled identification of endothelium. These findings suggest that endothelialization of synthetic arterial grafts may occur more frequently in human beings than previously recognized. |
| Databáze: | OpenAIRE |
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