A New Type of Modified Brassinosteroids for Enzyme-linked Immunosorbent Assay

Autor:	R. P. Litvinovskaya, A. G. Pryadko, Vladimir A. Khripach, Bernd Schneider, Tatyana Novik, O. V. Sviridov, Vladimir N. Zhabinskii, S. V. Drach, A. V. Antonchick, Vitaliy Matveentsev
Rok vydání:	2008
Předmět:	Pharmacology chemistry.chemical_classification Chromatography Enzyme Complementary and alternative medicine chemistry Drug Discovery Plant Science General Medicine Hapten
Zdroj:	Scopus-Elsevier
ISSN:	1555-9475 1934-578X
DOI:	10.1177/1934578x0800300513
Popis:	New modified brassinosteroids (BS) with a full set of specific functional groups and with an additional one at C-26 have been synthesized and used as haptens for enzyme-linked immunosorbent assay. The haptens were conjugated to bovine serum albumin (BSA) and the obtained immunoconjugates were applied for production of polyclonal antibodies. A conjugation of the haptens to horseradish peroxidase (HRP) gave the corresponding enzyme-labeled BS – another principal component for immuno-competitive analysis. The steroids were linked to proteins through spacers at the terminal side chain carbon atom (C-26) that made both the cyclic part and the side chain exposed for recognition by antibodies. It allowed maximization of their sensitivity and for the first time made possible immunochemical distinguishing of the most important group of natural BS, B-lactones, from B-ketones and 6-deoxo derivatives. The synthesis of haptens based on a sequence of transformations starting from stigmasterol included Claisen rearrangement of 22-allylic ethers as a key reaction in the side chain construction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85e1788cd8bab41197f0847410fa9deb https://doi.org/10.1177/1934578x0800300513 Zobrazit plný text záznamu